Abstract
A pair of proper primers were designed and synthesized for Polymerase Chain Reaction (PCR) of anti-bacterial gene aiiA which encodes protein aiiA from marine bacterial genome, according to the gene sequences of aiiA. The plasmid pMD18-ZD02aiiA was constructed after PCR testing of aiiA gene. The gene sequence of aiiA was amplified with proper primers (with active locations by enzymes BamHIand EcoRI) from molding board of pMD18-ZD02aiiA and linked into expression vector pET-17b after it was hydrolyzed by enzymes of BamHI and EcoRI. And then plasmids pET-ZD02aiiA was constructed. The results of hydrolyzation of enzymes, PCR amplification and sequencing demonstrated that the target gene fragments were inserted into vector pET-17b correctly, the sequences of gene aiiA and frame of reading codes were right. Thus, it may provide base for the recombinant express and induced express of anti-bacterial gene from marine bacterium.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Fuqua WC, Winans SC, Greenberg EP (1994) Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators. J Bacteriol 176(2):269–275
Fugua WC, Winans SC, Greenberg EP (1996) Census and consensus in bacterial ecosystems: the LuxR-Luxl family of quorum. Sensing transcriptional regulators. Annu Rev Microbiol 50:727–751
Choi SH, Greenberg EP (1992) Genetic dissection of DNA binding and luminescence gene activation by the Vibrio fischeri LuxR Protein. J Bacteriol 174(12):4064–4069
Nealson KH, Hastings JW (1979) Bacterial bioluminescence: its control and ecological significance. Microbiol Rev 43(4):496–518
Ding X, Yin B, Qian L et al (2011) Screening for novel quorum-sensing inhibitors to interfere with the formation of P. aeruginosa biofilm. J Med Microbiol 60(12):1827–1834
Pearson JP, Gray KM, Passador L et al (1994) The structure of the autoinducer required for expression of Pseudomonas aeruginosa virulence genes. Proc Natl Acad Sci USA 91(1):197–201
Swift S, Downie JA, Whitehead NA et al (2001) Quorum sensing as a population-density-dependent determinant of bacterial physiology. Adv Microb Physiol 45:199–270
Dong YH, Zhang LH (2005) Quorum sensing and quorum-quenching enzymes. J Microbiol 43:101–109
Acknowledgments
This work was supported by grants from Science and Technology Plan Project of Guangdong Province (2009B020311003), Science and Technology Plan Project of Jiangsu Province (BN2010038), Special Scientific Research Funds for Central Non-profit Institutes, Chinese Academy of Fishery Sciences (2010TS01), Production, Learning and Research Project of Guangdong Province (2011B090400151).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer-Verlag London
About this paper
Cite this paper
Ding, X., Yin, B., Zhang, S., Tang, W., Sun, W., Zhou, S. (2013). Construction of Recombinant Expression Vector of Anti-Bacterial Gene aiiA from Marine Bacterium. In: Du, W. (eds) Informatics and Management Science V. Lecture Notes in Electrical Engineering, vol 208. Springer, London. https://doi.org/10.1007/978-1-4471-4796-1_28
Download citation
DOI: https://doi.org/10.1007/978-1-4471-4796-1_28
Published:
Publisher Name: Springer, London
Print ISBN: 978-1-4471-4795-4
Online ISBN: 978-1-4471-4796-1
eBook Packages: EngineeringEngineering (R0)