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Blueberry in Calcium- and Vitamin D-Enriched Fermented Milk Is Able to Modulate Bone Metabolism in Postmenopausal Women

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Nutritional Influences on Bone Health

Abstract

Research in the field of nutrition allows considering the establishment of a real prevention of osteoporosis. The value of fruits is discussed. Indeed, red fruits are particularly interesting for their high content in anthocyans, endowed with antioxidant and anti-inflammatory properties.

Fifty-six postmenopausal women (less than 6 years) aged 50–65 years, without HRT, were included in a controlled, randomized, double-blind placebo, prospective study, after a medical examination and a blood test. Throughout the 3-month study period, they kept their eating habits, limiting however consumption of red fruits. They were randomized into two groups of 28 subjects receiving either 0 or 120 mg of anthocyans daily, from blueberry extract. Those polyphenols were provided at the dose of 0 or 60 mg of active molecule in 100 ml of fermented milk (two bottles of 100 ml/day covering 25 and 20 % RDA for calcium and vitamin D, respectively).

Consumption of the milk enriched in polyphenols significantly improved serum bALP activity (an osteoblastic marker), without significant modification of CTX, a marker for bone resorption. This favorable orientation of bone metabolism could be explained by the contribution of anthocyans, the only noticeable difference between the two test foods. This finding is independent of the initial calcium and vitamin D consumption.

In conclusion, consumption of fermented milk enriched with calcium and ­vitamin D, containing blueberry, for 3 months, has corrected the insufficiency of vitamin D of postmenopausal women and resulted in improved bone formation, as indicated by the rise of a biomarker of osteoblastic activity. This benefit is probably related to the presence of blueberries (rich in polyphenols and phenolic acids).

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Abbreviations

bALP:

Bone alkaline ­phosphatase

CRP:

C-reactive protein

CTX:

C-telopeptide cross-links

DPD:

Deoxypyridinoline

IU:

International unit

25(OH)D:

25-hydroxyvitamin D

PYD:

Pyridinoline

RDA:

Recommended dietary allowance

SEM:

Standard error to the mean

References

  1. Seeman E. Pathogenesis of bone fragility in women and men. Lancet. 2002;359(9320):1841–50.

    Article  PubMed  Google Scholar 

  2. Maravic M, Le Bihan C, Landais P, Fardellone P. Incidence and cost of osteoporotic fractures in France during 2001. A methodological approach by the national hospital database. Osteoporos Int. 2005;16(12):1475–80.

    Article  PubMed  Google Scholar 

  3. Rizzoli R, Boonen S, Brandi ML, Burlet N, Delmas P, Reginster JY. The role of calcium and vitamin D in the management of osteoporosis. Bone. 2008;42(2):246–9.

    Article  PubMed  CAS  Google Scholar 

  4. Dew TP, Day AJ, Morgan MR. Bone mineral density, polyphenols and caffeine: a ressaissement. Nutr Res Rev. 2007;20:89–105.

    Article  PubMed  CAS  Google Scholar 

  5. Das AS, Mukherjee M, Mitra C. Evidence for a prospective anti-osteoporosis effect of black tea (Camellia sinensis) extract in a bilaterally ovariectomized rat model. Asia Pac J Clin Nutr. 2004;13(2):210–6.

    PubMed  Google Scholar 

  6. Horcajada MN, Coxam V. Flavonols and isoflavones prevent bone loss in the ovariectomized rat a model for postmenopausal osteoporosis. In: Burckard F, editor. Nutritional aspects of osteoporosis. San Diego: Academic; 2001. p. 325–40.

    Google Scholar 

  7. Arjmandi BH, Khalil DA, Lucas EA, et al. Dried plums improve indices of bone formation in postmenopausal women. J Womens Health Gend Based Med. 2002;11(1):61–8.

    Article  PubMed  Google Scholar 

  8. Hegarty VM, May HM, Khaw KT. Tea drinking and bone mineral density in older women. Am J Clin Nutr. 2000;71(4):1003–7.

    PubMed  CAS  Google Scholar 

  9. Wu X, Beecher GR, Holden JM, Haytowitz DB, Gebhardt SE, Prior RL. Concentrations of ­anthocyanins in common foods in the United States and estimation of normal consumption. J Agric Food Chem. 2006;54(11):4069–75.

    Article  PubMed  CAS  Google Scholar 

  10. Chen CF, Li YD, Xu Z. Chemical principles and bioactivities of blueberry. Yao Xue Xue Bao. 2010;45(4):422–9.

    PubMed  CAS  Google Scholar 

  11. Wauquier F, Leotoing L, Coxam V, Guicheux J, Wittrant Y. Oxidative stress in bone remodelling and disease. Trends Mol Med. 2009;15(10):468–77.

    Article  PubMed  CAS  Google Scholar 

  12. Chapuy MC, Preziosi P, Maamer M, et al. Prevalence of vitamin D insufficiency in an adult normal population. Osteoporos Int. 1997;7(5):439–43.

    Article  PubMed  CAS  Google Scholar 

  13. Souberbielle JC, Cormier C, Kindermans C, et al. Vitamin D status and redefining serum parathyroid hormone reference range in the elderly. J Clin Endocrinol Metab. 2001;86(7):3086–90.

    Article  PubMed  CAS  Google Scholar 

  14. Heaney RP. Vitamin D, and calcium interactions: functional outcomes. Am J Clin Nutr. 2008;88(2):541S–4.

    PubMed  CAS  Google Scholar 

  15. Holick MF. Vitamin D, deficiency. N Engl J Med. 2007;357(3):266–81.

    Article  PubMed  CAS  Google Scholar 

  16. Bischoff-Ferrari HA, Willett WC, Orav EJ, Lips P, Meunier PJ, Lyons RA, Flicker L, Wark J, Jackson RD, Cauley JA, Meyer HE, Pfeifer M, Sanders KM, Stähelin HB, Theiler R, Dawson-Hughes B. A pooled analysis of vitamin D dose requirements for fracture prevention. N Engl J Med. 2012;367(1):40–9.

    Article  PubMed  CAS  Google Scholar 

  17. Golombick T, Diamond T. The effect of a combined oral calcium and vitamin D supplement for treating mild to moderate vitamin D deficiency in postmenopausal women. Clin Interv Aging. 2008;3(1):183–6.

    PubMed  CAS  Google Scholar 

  18. Romagnoli E, Mascia ML, Cipriani C, et al. Short and long-term variations in serum calciotropic hormones after a single very large dose of ergocalciferol (vitamin D2) or cholecalciferol (vitamin D3) in the elderly. J Clin Endocrinol Metab. 2008;93(8):3015–20.

    Article  PubMed  CAS  Google Scholar 

  19. Khaw KT, Sneyd MJ, Compston J. Bone density parathyroid hormone and 25-hydroxyvitamin D concentrations in middle aged women. BMJ. 1992;305(6848):273–7.

    Article  PubMed  CAS  Google Scholar 

  20. Dawson-Hughes B, Dallal GE, Krall EA, Harris S, Sokoll LJ, Falconer G. Effect of vitamin D supplementation on wintertime and overall bone loss in healthy postmenopausal women. Ann Intern Med. 1991;115(7):505–12.

    PubMed  CAS  Google Scholar 

  21. Dawson-Hughes B, Harris SS, Dallal GE, Lancaster DR, Zhou Q. Calcium supplement and bone medication use in a US Medicare health maintenance organization. Osteoporos Int. 2002;13(8):657–62.

    Article  PubMed  CAS  Google Scholar 

  22. Di Daniele N, Carbonelli MG, Candeloro N, Iacopino L, De Lorenzo A, Andreoli A. Effect of supplementation of calcium and vitamin D on bone mineral density and bone mineral content in peri- and post-menopause women; a double-blind, randomized, controlled trial. Pharmacol Res. 2004;50(6):637–41.

    PubMed  Google Scholar 

  23. Amer M, Qayyum R. Relation between serum 25-hydroxyvitamin D and C-reactive protein in asymptomatic adults (from the continuous National Health and Nutrition Examination Survey 2001 to 2006). Am J Cardiol. 2012;109(2):226–30.

    Article  PubMed  CAS  Google Scholar 

  24. Srivastava AK, Vliet EL, Lewiecki EM, et al. Clinical use of serum and urine bone markers in the management of osteoporosis. Curr Med Res Opin. 2005;21(7):1015–26.

    Article  PubMed  CAS  Google Scholar 

  25. Garnero P. Biomarkers for osteoporosis management: utility in diagnosis, fracture risk prediction and therapy monitoring. Mol Diagn Ther. 2008;12(3):157–70.

    Article  PubMed  CAS  Google Scholar 

  26. MC Donald CJ, Fraser WD, Reid DM, MC Donald HM. Dietary anthocyanidin intakes are associated with increased bone mineral density and decreased markers of bone resorption in a population of Scottish women. In: 8th international symposium on nutritional aspects of osteoporosis (Isnao) proceedings, Lausanne; 2012, p. 35.

    Google Scholar 

  27. Devareddy L, Hooshmand S, Collins JK, Lucas EA, Chai SC, Arjmandi BH. Blueberry prevents bone loss in ovariectomized rat model of postmenopausal osteoporosis. J Nutr Biochem. 2008;19(10):694–9.

    Article  PubMed  CAS  Google Scholar 

  28. Sellappan S, Akoh CC, Krewer G. Phenolic compounds and antioxidant capacity of Georgia-grown blueberries and blackberries. J Agric Food Chem. 2002;50(8):2432–8.

    Article  PubMed  CAS  Google Scholar 

  29. Taruscio TG, Barney DL, Exon J. Content and profile of flavanoid and phenolic acid compounds in conjunction with the antioxidant capacity for a variety of northwest Vaccinium berries. J Agric Food Chem. 2004;52(10):3169–76.

    Article  PubMed  CAS  Google Scholar 

  30. Kamitani Y, Maki K, Tofani I, Nishikawa Y, Tsukamoto K, Kimura M. Effects of grape seed ­proanthocyanidins extract on mandibles in ­developing rats. Oral Dis. 2004;10(1):27–31.

    Article  PubMed  CAS  Google Scholar 

  31. Kojima K, Maki K, Tofani I, Kamitani Y, Kimura M. Effects of grape seed proanthocyanidins extract on rat mandibular condyle. J Musculoskelet Neuronal Interact. 2004;4(3):301–7.

    PubMed  CAS  Google Scholar 

  32. Ishikawa M, Maki K, Tofani I, Kimura K, Kimura M. Grape seed proanthocyanidins extract promotes bone formation in rat’s mandibular condyle. Eur J Oral Sci. 2005;113(1):47–52.

    Article  PubMed  CAS  Google Scholar 

  33. Chen JR, Lazarenko OP, Wu X, et al. Dietary-induced serum phenolic acids promote bone growth via p38 MAPK/beta-catenin canonical Wnt signaling. J Bone Miner Res. 2010;25(11):2399–411.

    Article  PubMed  CAS  Google Scholar 

  34. Zhang J, Lazarenko OP, Blackburn ML, et al. Feeding blueberry diets in early life prevent senescence of osteoblasts and bone loss in ovariectomized adult female rats. PLoS One. 2011;6(9):e24486.

    Article  PubMed  CAS  Google Scholar 

  35. Zhang J, Lazarenko OP, Blackburn ML, Badger TM, Ronis MJ, Chen JR. Blueberry consumption prevents loss of collagen in bone matrix and inhibits senescence pathways in osteoblastic cells. Age (Dordr). 2012; [Epub ahead of print].

    Google Scholar 

  36. Karlsen A, Retterstol L, Laake P, et al. Anthocyanins inhibit nuclear factor-kappaB activation in monocytes and reduce plasma concentrations of pro-inflammatory mediators in healthy adults. J Nutr. 2007;137(8):1951–4.

    PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Human Nutrition, INRA, UMR1019, UNH, CRNH Auvergne, F-63000, Clermont-Ferrand, France

    Marie-Jeanne Davicco PhD, Caroline Puel PhD, Patrice Lebecque PhD & Véronique Coxam

  2. Clermont Université, Université d’Auvergne, Unité de Nutrition Humaine, BP 10448, F-63000, Clermont-Ferrand, France

    Marie-Jeanne Davicco PhD, Caroline Puel PhD, Patrice Lebecque PhD & Véronique Coxam

  3. Thématique Nutrition, SOREDAB, La Boissière Ecole, Paris, France

    Caroline Puel PhD

Authors
  1. Marie-Jeanne Davicco PhD
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  2. Caroline Puel PhD
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  3. Patrice Lebecque PhD
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  4. Véronique Coxam
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Corresponding author

Correspondence to Véronique Coxam .

Editor information

Editors and Affiliations

  1. , Department of Internal Medicine, Hirslanden Clinic/Bois Cerf, Ave. d'Ouchy 31, Lausanne, 1006, Vaud, Switzerland

    Peter Burckhardt

  2. , Human Nutrition Research Center on Aging, Tufts University, 711 Washington Street, Boston, 02111, Massachusetts, USA

    Bess Dawson-Hughes

  3. , Nutrition and Science, Purdue University, W. State Street 700, West Lafayette, 47907-2059, Indiana, USA

    Connie M. Weaver

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Davicco, MJ., Puel, C., Lebecque, P., Coxam, V. (2013). Blueberry in Calcium- and Vitamin D-Enriched Fermented Milk Is Able to Modulate Bone Metabolism in Postmenopausal Women. In: Burckhardt, P., Dawson-Hughes, B., Weaver, C. (eds) Nutritional Influences on Bone Health. Springer, London. https://doi.org/10.1007/978-1-4471-2769-7_37

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  • DOI: https://doi.org/10.1007/978-1-4471-2769-7_37

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