Abstract
A brief introduction to the fundamental theory of fluorescence spectroscopy applied to bioaerosol detection is given and developed systems are described. Bioaerosol detection relies on the fact that many relevant microorganisms contain molecules such as aromatic amino acids and reduced nicotinamide adenine dinucleotide (NADH) with characteristic fluorescence when excited by ultraviolet (UV) radiation. Several bioaerosol detection systems based on fluorescence have been developed and tested during the last two decades. They have proven to be very sensitive with a short response time. The main drawback of fluorescence is the relatively low specificity. There are ways to increase the classification capability by utilizing multiple wavelengths, spectral and temporal detection of the emission. Some of the design considerations are presented, including choices of excitation sources and detectors. This chapter is concluded with an outlook for the future.
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Jonsson, P., Kullander, F. (2014). Bioaerosol Detection with Fluorescence Spectroscopy. In: Jonsson, P., Olofsson, G., Tjärnhage, T. (eds) Bioaerosol Detection Technologies. Integrated Analytical Systems. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-5582-1_7
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