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Large-scale chromatography of proteins

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Chromatography

Part of the book series: Advances in Biochemical Engineering ((ABE,volume 25))

Abstract

Basic chromatography theory and its implication for scaling-up is thoroughly discussed as well as the implication of flow resistance in compressible gel materials. The advantages and disadvantages of different column designs are discussed, e.g. their effect on the non-chromatographic zone spreading. The properties of various column packing materials are compared and procedures for their maintenance are described. The engineering aspects of the construction of production scale columns are treated in detail as well as the operational behaviour of bed packings and the design of column end pieces. Aspects on the choice of pipe diameter, valve types and pumps for process chromatography are discussed and arrangements for filters, sampling devices, pressure switches etc. are suggested. Automation of chromatographic processes is briefly discussed. Examples of industrial applications of gel filtration, ion exchange chromatography and affinity chromatography are given, primarily within the pharmaceutical industry (plasma protein fractionation, insulin purification etc.).

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Abbreviations

A:

cross sectional area

Across:

cross sectional area of a particle perpendicular to the direction of movement

a:

parameter (function of bed height, diameter and solvent regain)

D:

solute diffusion coefficient

Dc :

column diameter

dp :

particle diameter

d:

pipe diameter

Ffrict :

frictional force

ffrict :

friction coefficient

fD :

drag factor

g:

gravitational acceleration

H:

plate height

h:

height relative to a reference level

K:

permeability

K0 :

permeability extrapolated to zero pressure drop

L:

column length, pipe, length

N:

plate number

NRe :

Reynolds number

Pin :

inlet ambient pressure

Pout :

outlet ambient pressure

ΔPpump :

pump pressure head

ΔPfrict :

pressure drop due to frictional losses

ΔP:

pressure drop over gel or column

Rs :

resolution

R:

ratio of zone velocity to mobile phase velocity

r:

pipe radius

td :

time spent by solute between sprption and desorption

Up :

fall velocity of a particle in a fluid

u:

velocity of a fluid element in a particular location

umax :

velocity of a fluid element in the center of a tube

v:

velocity of the mobile phase

Ve :

elution volume

Vred :

reduced velocity

Vt :

total bed volume

W:

peak width between baseline intercepts

X:

distance from pipe wall

β:

pipe friction factor

η:

viscosity

ϱ:

density

σ:

standard deviation

ψ:

shape factor

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Authors and Affiliations

  1. Pharmacia Fine Chemicals AB, Uppsala, Sweden

    Jan-Christer Janson & Per Hedman

  2. Biochemical Separation Center, Uppsala University, Uppsala, Sweden

    Jan-Christer Janson & Per Hedman

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  1. Jan-Christer Janson
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  2. Per Hedman
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A. Fiechter

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Janson, JC., Hedman, P. (1982). Large-scale chromatography of proteins. In: Fiechter, A. (eds) Chromatography. Advances in Biochemical Engineering, vol 25. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3540118292_3

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  • DOI: https://doi.org/10.1007/3540118292_3

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