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Segmental Isotopic Labeling: Prospects for a New Tool to Study the Structure-function Relationships in Multi-domain Proteins

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Protein NMR for the Millennium

Part of the book series: Biological Magnetic Resonance ((BIMR,volume 20))

Abstract

Segmental isotopic labeling allows NMR active nuclei to be introduced selectively into a specific region(s) of full-length protein sequences. In this perspective we review briefly the technologies available for the segmental isotopic labeling of proteins and suggest how these strategies might be combined with current NMR techniques to study aspects of protein function that are often difficult to address using conventional structural analysis.

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Author information

Authors and Affiliations

  1. Laboratories of Synthetic Protein Chemistry, The Rockefeller University, 1230 York Avenue, New York, NY, 10021, USA

    Jennifer J. Ottesen, Ulrich K. Blaschke & Tom W. Muir

  2. Laboratory Physical Biochemistry, The Rockefeller University, 1230 York Avenue, New York, NY, 10021, USA

    David Cowburn

  3. New York Structural Biology Center, c/o Box 163, 1230 York Avenue, New York, NY, 10021

    David Cowburn

Authors
  1. Jennifer J. Ottesen
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  2. Ulrich K. Blaschke
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  3. David Cowburn
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  4. Tom W. Muir
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Editor information

Editors and Affiliations

  1. University of Alabama at Birmingham, Birmingham, Alabama

    N. Rama Krishna

  2. University of Denver, Denver, Colorado

    Lawrence J. Berliner

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© 2002 Kluwer Academic Publishers

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Ottesen, J.J., Blaschke, U.K., Cowburn, D., Muir, T.W. (2002). Segmental Isotopic Labeling: Prospects for a New Tool to Study the Structure-function Relationships in Multi-domain Proteins. In: Krishna, N.R., Berliner, L.J. (eds) Protein NMR for the Millennium. Biological Magnetic Resonance, vol 20. Springer, Boston, MA. https://doi.org/10.1007/0-306-47936-2_2

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  • DOI: https://doi.org/10.1007/0-306-47936-2_2

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-0-306-47448-4

  • Online ISBN: 978-0-306-47936-6

  • eBook Packages: Springer Book Archive

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