Abstract
Guanidinoethane sulfonic acid (GES), a chemical and biological analog of taurine, decreases rat muscle taurine content when added to drinking water. Over the same period, GES appears in muscle8. GES supplementation is often used to study the effect of taurine depletion on physiological mechanisms, without taking into account the possible actions of GES. The purpose of the present study was to investigate the specific actions of GES on contraction of skeletal muscle. In mice EDL muscle, the time delay needed to observe a 20% force decrease after the end of a tetanic stimulation was higher in GES-supplemented than in control muscle. This observation in GES-supplemented muscle could be explained by the action of taurine or GES on several targets, beside others the rate of Ca2+ uptake by sarcoplasmic reticulum (SR) and the Ca2+ sensitivity of myofilaments. SR of rat EDL was isolated by successive centrifugations. The effect of 20 mM taurine or GES on the rate of Ca2+ uptake by SR was measured with the fluorescent Ca2+ indicator fura-2. The results show that the rate of Ca2+ uptake by SR is not modified in the presence of taurine or GES. The Ca2+ sensitivity of myofilaments was studied in chemically skinned fibers in the presence of 20 mM taurine or GES. Both taurine and GES increased the myofilament sensitivity to Ca2+. Thus, the prolonged relaxation time of GES-supplemented muscle can be attributed to an increase in myofilament sensitivity to Ca2+. This higher sensitivity is not due to a decrease in muscle taurine content but rather to an increased GES concentration.
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References
Allen, J.D., and Moss, R.L., 1987, Factors influencing the ascending limb ofthe sarcomere length-tension relationship in rabbit skinned muscle fibres. J. Physiol. 390: 119–136.
Brenner, B., 1998, Muscle mechanics II: skinned muscle fibres. In Current methods in muscle physiology. Advantages, problems and limitations (H. Sugi, ed.), Oxford University Press, Oxford, pp.22–69.
Galler, S., Hutzler, C., and Haller, T., 1990, Effects of taurine on Ca2+-dependent force development of skinned muscle fibre preparations. J. Exp. Biol. 152: 255–264.
Ganapathy, V., and Leibach, F.H., 1994, Expression and regulation of the taurine transporter of cultured cell lines of human origin. In Taurine in Health and disease (R. Huxtable), Plenum Press, New York, pp.51–57.
Grynkiewicz, G., Poenie, M., and Tsien, R.Y., 1985, A new generation of Ca2+indicators with greatly improved fluorescent properties. J. Biol. Chem. 260:3440–3450.
Huxtable, R.J., 1992, Physiological actions of taurine. Physiol. rev. 72: 101–163.
Huxtable, R., and Bressler, R., 1973, Effect of taurine on a muscle intracellular membrane. Biochimi. Biophys. Acta 323: 573–583.
Huxtable, R.J., Laird, H.E., and Lippincott, S.E., 1979, The transport of taurine in the heart and the rapid depletion of tissue taurine content by guanidinoethyl sulfonate. J. Pharmacol. Exp. Ther. 211: 465–471.
Jacobsen J.G., and Smith L.H., 1968, Biochemistry and physiology of taurine and taurine derivatives. Physiol. Rev. 48: 424–511.
Kargacin, M.E., Scheid C.R., and Honeyman T.W., 1988, Continuous monitoring of Ca2+ uptake in membrane vesicules with fura-2. Am. J. Physiol. 245: C694–C698.
Lake, N., Splawinski, J.B., Juneai, C., and Rouleau, J.L., 1990, Effects of taurine depletion on intrinsic contractility of rat ventricular papillary muscles, Can. J Physiol. Pharmacol. 68: 800–806.
Mozaffari, M.S., Tan, B.H., Lucia M.A., and Schaffer S.W., 1986, Effect of drug-induced taurine depletion on cardiac contractility and metabolism, Biochem. Pharm. 35: 985–989.
O'Brien, P. J., 1990, Calcium sequestration by isolated sarcoplasmic reticulum: real-time monitoring using radiometric dual-emission spectrofluorometry and the fluorescent calcium-binding dye indo-l. Mol. Cell. Biochem. 94: 113–119.
Remtulla, M.A., Katz, S., and Applegarth D.A., 1978, Effect of taurine on ATP-dependent calcium transport in guinea-pig cardiac muscle. Life Sciences. 23: 383–390.
Steele, D.S., Smith, G.L., and Miller, D.J., 1990, The effects of taurine on calcium uptake by the sarcoplasmic reticulum and calcium sensitivity of chemically skinned rat heart. J Physiol. 422: 499–511.
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© 2002 Kluwer Academic Publishers
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Cuisinier, C., Gailly, P., Francaux, M., Lebacq, J. (2002). Effects of Guandinoethane Sulfonate on Contraction of Skeletal Muscle. In: Della Corte, L., Huxtable, R.J., Sgaragli, G., Tipton, K.F. (eds) Taurine 4. Advances in Experimental Medicine and Biology, vol 483. Springer, Boston, MA. https://doi.org/10.1007/0-306-46838-7_46
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DOI: https://doi.org/10.1007/0-306-46838-7_46
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