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Assessment of bacoside production, total phenol content and antioxidant potential of elicited and non-elicited shoot cultures of Bacopa monnieri (L.)

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Abstract

Thirteen accessions of Bacopa monnieri (L.) Wettst. were assessed for in vitro propagation, total bacosides, total phenol content and antioxidant potential. Nodal explants were cultured on Murashige and Skoog (MS) medium with 0.4 mg/L benzyl aminopurine (BA) to identify best accession based on growth parameters. The selected accession (IC 554588) was elicited with 1 mg/L of jasmonic acid, salicylic acid or malt extract to assess its efficacy in enhancing secondary metabolites production in in vitro cultures. Elicitor-treated plants showed increased production of bacosides (2.7–3.9 fold), total phenol content (5–18 fold) and higher antioxidant potential (7.9 fold) in 4 weeks. According to statistical analysis, antioxidant potential is highly correlated to total phenol as R-sq (adj) is 90.1%. Data was subjected to correlation, Principal Component Analysis (PCA) and cluster analysis to understand the relationship between different variables and identify major contributors of variability. Strong correlation between total bacoside, total phenol, and antioxidant activity indicate that elevated antioxidant potential was the result of overall increase in bacoside and phenol content, which can be enhanced by the application of elicitors. The study suggested that accessions IC 554588, IC 344312 and IC 554585 are elite and may be utilized for various pharmacopeias after further in vivo research and clinical trials.

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Acknowledgements

We are grateful to Vice Chancellor of Delhi Technological University, Delhi, India for constant support and encouragement in conducting this study. We are also thankful to Dr. Ram Singh, Assistant Professor (Applied Chemistry) and his advisee Dr. Atya and Dr. Gitika (Applied Chemistry), Delhi Technological University for their help in performing HPLC.

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This research did not receive any grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Department of Biotechnology, Delhi Technological University, Delhi, India

    Nupur Jauhari & Navneeta Bharadvaja

  2. ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, India

    Rakesh Bharadwaj & Neelam Sharma

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  1. Nupur Jauhari
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  2. Rakesh Bharadwaj
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  3. Neelam Sharma
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  4. Navneeta Bharadvaja
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Experimental strategy belongs to NB, NS and NJ. NJ and RB performed experiment. RB did statistical analysis.

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Correspondence to Navneeta Bharadvaja.

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Jauhari, N., Bharadwaj, R., Sharma, N. et al. Assessment of bacoside production, total phenol content and antioxidant potential of elicited and non-elicited shoot cultures of Bacopa monnieri (L.). Environmental Sustainability 2, 441–453 (2019). https://doi.org/10.1007/s42398-019-00071-3

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