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Inoculation of endophlytic diazotrophic bacteria in micropropagated seedlings of sugarcane (Saccharum officinarum sp.)

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Abstract

The inoculation of diazotrophic endophytic bacteria in micropropagated sugarcane plants has been utilized in studies on the association between plants and diazotrophic bacteria, allowing us to evaluate the potential of BNF and growth promotion. The objective of this study was to evaluate the effects of inoculation (alone and in a mixture) of different strains of endophytic bacteria from the sugarcane variety RB867515, collected in northeast Brazil, on sugarcane growth at the initial growth stage (45 and 120 days after inoculation—DAI). For this purpose, two experiments were carried out in a greenhouse at the Agronomic Institute of Pernambuco (IPA), located in the city of Goiania, PE, Brazil, in a completely randomized design. The first experiment, with micropropagated seedlings grown in tubes at 45 DAI, was composed of uninoculated plants, plants inoculated in vitro with three individual endophytic bacterial isolates, and plants inoculated in vitro with a mixture of all three bacterial isolates. The second experiment, at 150 DAI, consisted of inoculated plants transplanted to pots with nonsterile soil without nitrogen fertilization and uninoculated plants with nitrogen fertilization equivalent to 80 kg of N ha−1. The variables analyzed were the shoot and root dry weight, tillering and N content accumulated in the plant. At 45 DAI, there was no significant difference between the inoculated plants and the uninoculated control. The inoculation of nitrogen-fixing bacteria native to the northeast region in micropropagated seedlings of sugarcane variety RB867515 grown in pots promoted plant development and presented similar performance to the nitrogen treatment at 150 DAI.

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Funding

This work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq grant number 310030/2015-3), and MCSB obtained a scholarship from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).

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Authors and Affiliations

  1. Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Av. Prof. Morais Rego, S/N, Cidade Universitária, Recife, Pernambuco, CEP 50670-420, Brazil

    Maria do Carmo Silva Barreto, Márcia Vanusa Silva, Clébia Maria Alves Almeida & Vera Lúcia de Menezes Lima

  2. Laboratório de Biologia do Solo, Instituto Agronômico de Pernambuco, Av. General San Martin, 1371, Bongi, Recife, Pernambuco, CEP 50761-000, Brazil

    Márcia do Vale Barreto Figueiredo, José de Paula de Oliveira & Manoel Urbano Ferreira Junior

  3. Departamento de Química, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, S/N, Dois Irmãos, Recife, Pernambuco, CEP 52171-900, Brazil

    Arnóbio Gonçalves Andrade & Carolina Etiene Rosália e Silva Santos

  4. Centro de Tecnologias Estratégicas do Nordeste, Av. Prof. Luís Freire, 1, Cidade Universitária, Recife, Pernambuco, CEP, 50740-540, Brazil

    Odemar Vicente dos Reis Junior

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  1. Maria do Carmo Silva Barreto
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  2. Márcia do Vale Barreto Figueiredo
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  3. Márcia Vanusa Silva
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  4. José de Paula de Oliveira
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  5. Arnóbio Gonçalves Andrade
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  6. Clébia Maria Alves Almeida
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  7. Manoel Urbano Ferreira Junior
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  8. Carolina Etiene Rosália e Silva Santos
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  9. Odemar Vicente dos Reis Junior
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  10. Vera Lúcia de Menezes Lima
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Correspondence to Márcia do Vale Barreto Figueiredo or Vera Lúcia de Menezes Lima.

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do Carmo Silva Barreto, M., do Vale Barreto Figueiredo, M., Silva, M.V. et al. Inoculation of endophlytic diazotrophic bacteria in micropropagated seedlings of sugarcane (Saccharum officinarum sp.). Environmental Sustainability 2, 5–12 (2019). https://doi.org/10.1007/s42398-019-00044-6

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