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Physiological, biochemical and molecular responses of lentil (Lens culinaris Medik.) genotypes under drought stress

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Abstract

Lentil (Lens culinaris Medik.) is an important pulse crop in India. It is moderately tolerant to drought, however intermittent and terminal drought significantly reduce lentil productivity. Selection of appropriate parent for breeding drought resistant variety is a challenging task. Thus, in the present work drought response of eight lentil genotypes (GP3690, LL1136, GP3643, NDL908, KLS218, IC248956, PL230, L4076) has been analysed, by imposing drought stress using PEG 6000 (18% w/v) for 15 days. Various physiological (stomatal density, relative water content) and biochemical parameters (total chlorophyll, total soluble sugar, anthocyanin and proline contents, lipid peroxidation, superoxide dismutase and catalase activities) were analysed under drought stress. These eight genotypes were further evaluated by analysing expression of drought stress marker genes (DREBs and RDs) which suggested genotypes GP3690 as drought susceptible (DS) and genotypes GP3643 and IC248956 as drought tolerant (DT). Relative expression of forty-three drought responsive genes related to various molecular functions, like biosynthetic process, redox homeostasis and defence related genes were evaluated in these three genotypes, which revealed that the tolerant genotypes alters the metabolic and biosynthesis processes of plant to overcome drought. This study provides basic information on drought tolerance capacity of the genotypes which may be further ascertained at field level.

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Acknowledgements

RS acknowledges Science and Engineering Research Board, Department of Science and Technology, Government of India for the National-Postdoctoral Fellowship (PDF/2016/000924). AKS acknowledges Institute projects IXX12585 and IXX12644 funded by ICAR-Indian Institute of Agricultural Biotechnology, Ranchi. We thank Dr. Madhuparna Banerjee, Associate Professor, College of Biotechnology, Birsa Agricultural Biotechnology, Ranchi for granting access to her lab facilities during the course of this study.

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  1. ICAR-Indian Institute of Agricultural Biotechnology, Garh Khatanga, Ranchi, 834 010, India

    Ragini Sinha & Anil Kumar Singh

  2. Department of Biochemistry and Crop Physiology, Bihar Agricultural University, Sabour, Bhagalpur, 813210, India

    Awadhesh Kumar Pal

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Sinha, R., Pal, A.K. & Singh, A.K. Physiological, biochemical and molecular responses of lentil (Lens culinaris Medik.) genotypes under drought stress. Ind J Plant Physiol. 23, 772–784 (2018). https://doi.org/10.1007/s40502-018-0411-7

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