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GhKLCR1, a kinesin light chain-related gene, induces drought-stress sensitivity in Arabidopsis

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Abstract

Drought stress results in significant losses in agricultural production, and especially that of cotton. The molecular mechanisms that coordinate drought tolerance remain elusive in cotton. Here, we isolated a drought-response gene GhKLCR1, which is a close homolog of AtKLCR1, which encodes a kinesin light chain-related protein enriched with a tetratrico peptide-repeat region. A subcellular localization assay showed that GhKLCR1 is associated with the cell membrane. A tissue-specific expression profile analysis demonstrated that GhKLCR1 is a cotton root-specific gene. Further abiotic and hormonal stress treatments showed that GhKLCR1 was upregulated during abiotic stresses, especially after polyethylene glycol treatments. In addition, the glucuronidase (GUS) staining activity increased as the increment of mannitol concentration in transgenic Arabidopsis plants harboring the fusion construct PGhKLCR1::GUS. The root lengths of 35S::GhKLCR1 lines were significantly reduced compared with that of wild type. Additionally, seed germination was strongly inhibited in 35S::GhKLCR1 lines after 300-mmol L−1 mannitol treatments as compared with Columbia-0, indicating the sensitivity of GhKLCR1 to drought. These findings provide a better understanding of the structural, physiological and functional mechanisms of kinesin light chain-related proteins.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31501345).

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Authors and Affiliations

  1. Xinjiang Research Base, State Key Laboratory of Cotton Biology, Xinjiang Agricultural University, Urumqi, 830052, China

    Jie Li, Daoqian Yu, Xiaodong Liu & Quanjia Chen

  2. State Key Laboratory of Cotton Biology, Key Laboratory of Biological and Genetic Breeding of Cotton, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, China

    Jie Li, Daoqian Yu, Ghulam Qanmber, Lili Lu, Lingling Wang, Lei Zheng, Zhao Liu, Huanhuan Wu, Fuguang Li & Zuoren Yang

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  11. Fuguang Li
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  12. Zuoren Yang
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Correspondence to Fuguang Li or Zuoren Yang.

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Li, J., Yu, D., Qanmber, G. et al. GhKLCR1, a kinesin light chain-related gene, induces drought-stress sensitivity in Arabidopsis. Sci. China Life Sci. 62, 63–75 (2019). https://doi.org/10.1007/s11427-018-9307-y

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  • DOI: https://doi.org/10.1007/s11427-018-9307-y

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