Abstract
The fluorescence yield of isolated fucoxanthin chlorophyll proteins, serving as light harvesting proteins in diatoms, was compared to the amount of diatoxanthin bound. Diatoxanthin was earlier shown to be involved in the xanthophyll cycle in diatoms as a functional analogue of zeaxanthin in higher plants. By growing cells under different light conditions, the amount of diatoxanthin in both the trimeric FCPa as well as the oligomeric FCPb of the diatom Cyclotella meneghiniana was increased. In the trimeric FCPa, the fluorescence yield decreased with increasing diatoxanthin content, whereas in the oligomeric FCPb fluorescence was generally lower, albeit constant. No pH dependence of fluorescence yield could be demonstrated except for artificially aggregated FCPa. Thus, diatoxanthin is able to quench fluorescence in FCPa, but the yield is also influenced by pH when the protein becomes aggregated.
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Abbreviations
- Chl:
-
Chlorophyll
- DDM:
-
ß-Dodecyl maltoside
- Dd:
-
Diadinoxanthin
- Dt:
-
Diatoxanthin
- FCP:
-
Fucoxanthin chlorophyll protein
- FP:
-
Free pigment
- HL:
-
High light
- HPLC:
-
High pressure liquid chromatography
- LHC:
-
Light harvesting complex
- LL:
-
Low light
- NPQ:
-
Non-photochemical quenching
- SDS-PAGE:
-
Sodium dodecyl sulfate polyacrylamide gel electrophoresis
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Acknowledgements
We gratefully acknowledge the support for this work by Marie Curie Training and Research Network (Intro2, MRTN-CT-2003-505069) of the European Community.
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Gundermann, K., Büchel, C. The fluorescence yield of the trimeric fucoxanthin–chlorophyll–protein FCPa in the diatom Cyclotella meneghiniana is dependent on the amount of bound diatoxanthin. Photosynth Res 95, 229–235 (2008). https://doi.org/10.1007/s11120-007-9262-1
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DOI: https://doi.org/10.1007/s11120-007-9262-1