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A novel polymorphism of GDF5 gene and its association with body measurement traits in Bos taurus and Bos indicus breeds

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Abstract

Body measurement traits, influenced by genes and environmental factors, play numerous important roles in the value assessment of productivity and economy. Growth differentiate factor 5 (GDF5), involved in the development and maintenance of bone and cartilage, is an important candidate gene for body measurement traits selection through marker-assisted selection (MAS). In this study, based on the PCR-RFLP technology, we discovered and evaluated the potential association of the single nucleotide polymorphism (SNP) (T586C in exon 1) of the bovine GDF5 gene with body measurement traits in 985 Bos taurus breed, 42 Bos indicus breed and 76 Bos indicus × Bos taurus individuals. As the SNP marker, there were the significant effects on the Body length (BL) in the Bos taurus (BT) and Bos indicus × Bos taurus (BMY) populations (P < 0.05). In BT population, animals with the genotype TT had lower mean values for BL and Hip width (HW) than these with the TC and CC genotype (P < 0.01). In BMY population, animals with the genotype TC had lower mean values for BL than these with the genotype CC (P < 0.05). These results suggest that the SNP of the GDF5 gene could be a very useful genetic marker for body measurement traits in the bovine reproduction and breeding.

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Acknowledgments

This work was supported by the China National “863” Program (2006AA10Z1A1), the National Eleventh “Five-Year” Science and Technology Support Project (2006BAD01A10-3), the “13115” Scientific and Technological Innovation Program of Shaanxi Province (2007ZDCY-01), National 973 Program (2006CB102105) and Talent Foundation for Northwest A&F University. Moreover, the bovine populations were supported by Qinchuan beef cattle breeding center of Shaanxi province, Nanyang, Jiaxian and Xianan cattle breeding center of Henan province, Luxi cattle breeding center of Shandong province and Yunnan beef cattle & pasture research center (P. R. China).

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Authors and Affiliations

  1. College of Animal Science and Technology, Northwest A & F University, No. 22 Xinong Road, 712100, Yangling, Shaanxi, People’s Republic of China

    Yong Feng Liu, Lin Sen Zan, Shuan Ping Zhao, Ya Ping Xin, Qing Lin & Wan Qiang Tian

  2. Key Laboratory for Farm Animal Genetic Resources and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Sciences, 100193, Beijing, People’s Republic of China

    Kui Li & Zhi Wei Wang

  3. National Beef Cattle Improvement Centre of China, 712100, Yangling, Shaanxi, People’s Republic of China

    Lin Sen Zan

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  1. Yong Feng Liu
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  2. Lin Sen Zan
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Correspondence to Lin Sen Zan.

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Liu, Y.F., Zan, L.S., Li, K. et al. A novel polymorphism of GDF5 gene and its association with body measurement traits in Bos taurus and Bos indicus breeds. Mol Biol Rep 37, 429–434 (2010). https://doi.org/10.1007/s11033-009-9604-5

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  • DOI: https://doi.org/10.1007/s11033-009-9604-5

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