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Comparison of the Effects of Aerobic Conditioning Before and After Pulmonary Allergic Inflammation

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Abstract

The aim of this study is to compare the effects of aerobic conditioning (AC) before (ACBS) and after (ACAS) allergic sensitization. BALB/c mice were divided into two main groups: ACBS and ACAS. Each groups was divided into subgroups: control (nonsensitized/nontrained), AC (nonsensitized/trained), ovalbumin (OVA) (sensitized/nontrained), AC + OVA (trained/sensitized), and OVA + AC (sensitized/trained). Sensitization was induced using OVA and AC performed in treadmill (moderate intensity). We examined IgE and IgG1 levels, eosinophil counting, expression of Th1 (interleukin (IL)-2, IFN-α) and Th2 cytokines (IL-4, IL-5, IL-13), IL-10, vascular endothelial growth factor (VEGF), and airway remodeling. IgE and IgG1 were decreased only when exercise was performed before sensitization (ACBS); however, there was a decrease of eosinophils, Th2 cytokines, VEGF, and airway remodeling and increase in IL-10 in either ACBS or ACAS groups. Our results demonstrate that aerobic conditioning reduces Th2 response before and after sensitization by increasing IL-10 while the production of anaphylactic antibodies is reduced only when exercise is performed before sensitization.

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Acknowledgments

This work was financially supported by the Fundação de Amparo a Pesquisa de São Paulo (FAPESP) grants 09/53904-9 and 2009/54420-5, the Conselho Nacional de Pesquisa (CNPq; grant 305987/2010-0), and the Laboratório de Investigação Médica 20 (LIM-20) of School of Medicine, University of São Paulo.

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Authors and Affiliations

  1. Department of Physical Therapy, University of Sao Paulo, São Paulo, Brazil

    Ronaldo Aparecido da Silva & Celso Ricardo Fernandes Carvalho

  2. Laboratory of Experimental Therapeutics LIM-20, School of Medicine, University of Sao Paulo, Av. Dr. Arnaldo, 455, Room 1210, 01246-903, São Paulo, SP, Brazil

    Ronaldo Aparecido da Silva, Francine Maria Almeida, Clarice Rosa Olivo, Beatriz Mangueira Saraiva-Romanholo, Adenir Perini, Milton Arruda Martins & Celso Ricardo Fernandes Carvalho

  3. University City of São Paulo (UNICID), São Paulo, Brazil

    Beatriz Mangueira Saraiva-Romanholo

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  1. Ronaldo Aparecido da Silva
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Correspondence to Celso Ricardo Fernandes Carvalho.

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da Silva, R.A., Almeida, F.M., Olivo, C.R. et al. Comparison of the Effects of Aerobic Conditioning Before and After Pulmonary Allergic Inflammation. Inflammation 38, 1229–1238 (2015). https://doi.org/10.1007/s10753-014-0090-0

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