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Stomatal NH3 compensation point and its metabolic regulation in senescence phenotypes of Nicotiana tabacum

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Biologia Plantarum

Abstract

We compared stomatal ammonia compensation point (χs) and its metabolic regulation in tobacco (Nicotiana tabacum) leaves of a quick-leaf-senescence phenotype ZY90 and a slow-leaf-senescence phenotype NC89. Compared with NC89, ZY90 had significantly higher χs values between 40 and 60 d after leaf sprouting in spite of its lower nitrogen content. During the same time, a steeper decline in glutamine synthetase activity was detected in ZY90 leaves, simultaneously with a steep increase in χs. These results suggested that the quick leaf senescence phenotype exhibited high NH3 emission potential due to efficient nitrogen recycling and remobilization, and glutamine synthetase played a key role in regulating χs in ZY90.

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Abbreviations

DAS:

days after leaf sprouting

GDH:

glutamate dehydrogenase

GS:

glutamine synthetase

[H+]apo :

apoplastic H+ content

[NH4 +]apo :

apoplastic NH4 + content

pHapo :

apoplastic pH

χs :

ammonia stomatal compensation point

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Authors and Affiliations

  1. Henan Agricultural University, Zhengzhou, 450002, P.R.China

    W. J. Duan, T. Z. Yang, X. Q. Zhang & H. B. Liu

  2. Research and Development Center, China Tobacco Chuanyu Industrial Corporation, Chengdu, 610066, P.R. China

    W. J. Duan, Y. Dai, D. L. Li, N. Li & C. G. Wang

Authors
  1. W. J. Duan
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  2. T. Z. Yang
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  3. Y. Dai
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  4. D. L. Li
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  5. X. Q. Zhang
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  6. H. B. Liu
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  7. N. Li
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  8. C. G. Wang
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Corresponding author

Correspondence to T. Z. Yang.

Additional information

Acknowledgements: This work was supported by grants from China Tobacco Chuanyu Industrial Corporation (Grant No. 2010-347-20).

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Duan, W.J., Yang, T.Z., Dai, Y. et al. Stomatal NH3 compensation point and its metabolic regulation in senescence phenotypes of Nicotiana tabacum . Biol Plant 56, 771–774 (2012). https://doi.org/10.1007/s10535-012-0141-z

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  • DOI: https://doi.org/10.1007/s10535-012-0141-z

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