Abstract
In this study the reproductive capacity of a laboratory-selected spirodiclofen resistant strain was investigated after treatment with spirodiclofen. Firstly, females were exposed to different concentrations of spirodiclofen (200 and 1,000 mg/l) during 6, 12 or 24 h. In contrast to the susceptible parental strain, the fecundity and fertility of resistant mites was not affected by treatment with these concentrations after any time of exposure tested. Secondly, pre-treatment of the resistant females with the synergists PBO or DEF could increase the inhibitory effect of spirodiclofen on reproduction, demonstrating the possible involvement of monooxygenases and esterases in metabolic detoxification of the acaricide. Because spirodiclofen interferes with lipid biosynthesis, total lipid content was measured in female adults. There were no significant differences between treated and non-treated female adults, both in the susceptible and resistant strain. However, the total lipid content in the resistant females was significantly higher than in susceptible females. Our data shows that the detection of spirodiclofen resistance should not be limited to mortality bioassays with eggs or larvae, but should be combined with inhibitory studies on female fertility and fecundity.
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References
Bretschneider T, Fischer R, Nauen R (2007) Inhibitors of lipid synthesis (acetyl-CoA-carboxylase inhibitors). In: Krämer W, Schirmer U (eds) Modern crop protection compounds. Wiley, Weinheim, pp 909–925
Dekeyser MA (2005) Acaricide mode of action. Pest Manag Sci 61:103–110. doi:10.1002/ps.994
Marcic D (2007) Sublethal effects of spirodiclofen on life history and life-table parameters of two-spotted spider mite (Tetranychus urticae). Exp Appl Acarol 42:121–129. doi:10.1007/s10493-007-9082-1
Nauen R (2005) Spirodiclofen: mode of action and resistance risk assessment in tetranychid pest mites. J Pestic Sci 30:272–274
Rauch N, Nauen R (2002) Spirodiclofen resistance risk assessment in Tetranychus urticae (Acari: Tetranychidae): a biochemical approach. Pestic Biochem Physiol 74:91–101. doi:10.1016/S0048-3575(02)00150-5
Sabelis MW (1985) Reproductive strategies. In: Helle W, Sabelis MW (eds) Spider mites, their biology, natural enemies and control, vol 1A. Elsevier, Amsterdam, pp 265–278
Van Handel E (1985) Rapid determination of total lipids in mosquitoes. J Am Mosq Control Assoc 1:302–304
Van Laecke K, Degheele D (1993) Effect of insecticide synergist combinations on the survival of Spodoptera exigua. Pestic Sci 37:283–288. doi:10.1002/ps.2780370308
Van Leeuwen T, Stillatus V, Tirry L (2004) Genetic analysis and cross-resistance spectrum of a laboratory-selected chlorfenapyr resistant strain of two-spotted spider mite (Acari: Tetranychidae). Exp Appl Acarol 32:249–261. doi:10.1023/B:APPA.0000023240.01937.6d
Van Pottelberge S, Van Leeuwen T, Khajehali J, Tirry L (2008) Genetic and biochemical analysis of a laboratory-selected resistant strain of Tetranychus urticae Koch (Acari: Tetranychidae). Pest Manag Sci. doi:10.1002/ps.1698
Wachendorff U, Nauen R, Schnorbach HJ, Rauch N, Elbert A (2002) The biological profile of spirodiclofen (Envidor®)—a new selective tetronic acid acaricide. Pflanzenschutz-Nachrichten Bayer 55:149–176
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Van Pottelberge, S., Khajehali, J., Van Leeuwen, T. et al. Effects of spirodiclofen on reproduction in a susceptible and resistant strain of Tetranychus urticae (Acari: Tetranychidae). Exp Appl Acarol 47, 301–309 (2009). https://doi.org/10.1007/s10493-008-9226-y
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DOI: https://doi.org/10.1007/s10493-008-9226-y