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Impact of tree species on barley rhizosphere-associated fungi in an agroforestry ecosystem as revealed by 18S rDNA PCR-DGGE

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Abstract

Agroforestry systems have been considered a form of sustainable land use. Woody species in agroforestry systems can improve soil physicochemical properties by supplying leaf or stem litter. However, little is known about fungal community structure and diversity in agroforestry systems. In the present study, the culture-independent 18S rDNA-based polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) method was used to investigate fungal community structure in rhizosphere and bulk soil in Populus euramevicana-barley and Taxodium distichum-barley agroforestry systems. DGGE profiling and cluster analysis revealed that the fungal community structure in the rhizosphere was more complex than that of bulk soil. Our results also indicated that the rhizosphere fungal community in barley was less affected by T. distichum than by P. euramevicana. In addition, an increase in the relative abundance of certain rhizosphere fungal populations was detected in this agroforestry system. Sequencing of prominent DGGE bands revealed an increase in the rhizosphere of a fungal species belonging to the genera Chaetomium, which includes potential biocontrol agents. A rare cellulolytic fungus, Acremonium alcalophilum, was found in the bulk soil from P. euramevicana and barley grown under P. euramevicana. Taken together, our findings may provide new insights into agroforestry practices.

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Acknowledgments

This study was funded by the National Forestry Public Welfare Industry Research Project (201304211), the Collaborative Innovation Plan of Jiangsu Higher Education, the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and Central Government Funding of Forestry Science and Technology Demonstration Projects ([2012]TJS1). The authors are grateful to the anonymous reviewers for their valuable suggestions.

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    Authors and Affiliations

    1. Co-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, 159 Longpan Road, Nanjing, 210037, China

      Qiang Zhang, Peng Zhou & Yanming Fang

    2. Jiangsu Academy of Forestry, Dong Shanqiao, Nanjing, 211153, China

      Min Zhang & Yonghua Ji

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    1. Qiang Zhang
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    2. Min Zhang
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    Correspondence to Yanming Fang.

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    Qiang Zhang and Min Zhang have contributed equally to this work.

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    Zhang, Q., Zhang, M., Zhou, P. et al. Impact of tree species on barley rhizosphere-associated fungi in an agroforestry ecosystem as revealed by 18S rDNA PCR-DGGE. Agroforest Syst 92, 541–554 (2018). https://doi.org/10.1007/s10457-017-0086-5

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