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Critical Comparison of Liquid Chromatography Coupled to Mass Spectrometry and Three Different Ion Mobility Spectrometry Systems on Their Separation Capability for Small Isomeric Compounds

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A Correction to this article was published on 05 July 2019

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Abstract

The very fast separation and identification of isomeric small substances with a molecular weight under 800 Da is still a challenge for high-throughput analysis. Inadequate chromatographic or spectrometric separation hampers an unequivocal identification of isomers, which may be recorded as a sum parameter. Reversed-phase liquid chromatography can be considered a generic method for the separation of isomers. However, a separation is usually achieved within minutes and not milliseconds as is typical for ion mobility spectrometry. The aim of this study, therefore, was to investigate the potential of IMS to separate small isomeric compounds. Hence, 23 substances divided into 11 isomeric groups have been selected. Among them, cancer drugs, hormones, pain relievers and others are contained. Three ion mobility spectrometers with different separation principles were compared with respect to their resolving performance. These systems comprised a traveling wave ion mobility spectrometer, a differential ion mobility spectrometer and a differential mobility analyzer. For reference, the chromatographic resolution and peak capacity was determined by high-performance liquid chromatography using a reversed phase.

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  • 05 July 2019

    Unfortunately a second footnote (Published in the topical collection 24th International Symposium on Separation Sciences …) was added to the original submission.

References

  1. O’Shaughnessy J, Miles D, Vukelja S et al (2002) Superior survival with capecitabine plus docetaxel combination therapy in anthracycline-pretreated patients with advanced breast cancer: phase III trial results. J Clin Oncol 20:2812–2823. https://doi.org/10.1200/JCO.2002.09.002

    Article  CAS  PubMed  Google Scholar 

  2. Monsuez JJ, Charniot JC, Vignat N, Artigou JY (2010) Cardiac side-effects of cancer chemotherapy. Int J Cardiol 144:3–15. https://doi.org/10.1016/j.ijcard.2010.03.003

    Article  PubMed  Google Scholar 

  3. Kaklamani VG, Gradishar WJ (2003) Epirubicin versus doxorubicin: which is the anthracycline of choice for the treatment of breast cancer? Clin Breast Cancer 4(suppl 1):S26–S33. https://doi.org/10.3816/CBC.2003.s.012

    Article  CAS  PubMed  Google Scholar 

  4. European Commission (2015) Commission Implementing Regulation (EU) 2015/495 of 20 March 2015 establishing a watch list of substances for Union-wide monitoring in the field of water policy pursuant to Directive 2008/105/EC of the European Parliament and of the Council. Off J Eur Union L78/40:20–30. http://eur-lex.europa.eu/pri/en/oj/dat/2003/l_285/l_28520031101en00330037.pdf. Accessed 15 June 2018

  5. Aalberg L, Clark CR, Deruiter J (2004) Chromatographic and mass spectral studies on isobaric and isomeric substances related to 3,4-methylenedioxymethamphetamine. J Chromatogr Sci 42:464–469. https://doi.org/10.1093/chromsci/42.9.464

    Article  CAS  PubMed  Google Scholar 

  6. Kocic D, Farrell W, Dennis GR, Shalliker RA (2016) Ultra-fast HPLC MS analyses using active flow technology columns. Microchem J 127:160–164. https://doi.org/10.1016/j.microc.2016.03.002

    Article  CAS  Google Scholar 

  7. Hetzel T, Blaesing C, Jaeger M et al (2017) Characterization of peak capacity of microbore liquid chromatography columns using gradient kinetic plots. J Chromatogr A 1485:62–69. https://doi.org/10.1016/j.chroma.2017.01.018

    Article  CAS  PubMed  Google Scholar 

  8. Eiceman GA, Karpas Z, Hill Herbert H, Jr (2016) Ion mobility spectrometry, 3rd edn. CRC Press, Boca Raton, Florida

    Google Scholar 

  9. Pu Y, Ridgeway ME, Glaskin RS et al (2016) Separation and identification of isomeric glycans by selected accumulation-trapped ion mobility spectrometry-electron activated dissociation tandem mass spectrometry. Anal Chem 88:3440–3443. https://doi.org/10.1021/acs.analchem.6b00041

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Mason EA, McDaniel EW (1988) Transport properties of ions in gases. Wiley, New York

    Book  Google Scholar 

  11. Kasner E, Hunter CA, Ph D et al (2013) The influence of drift gas composition on the separation mechanism in traveling wave ion mobility spectrometry: insight from electrodynamic simulations. Int J Ion Mob Spectrom 70:646–656. https://doi.org/10.1002/ana.22528.Toll-like

    Article  Google Scholar 

  12. Shvartsburg AA, Li F, Tang K, Smith RD (2006) High-resolution field asymmetric waveform ion mobility spectrometry using new planar geometry analyzers. Anal Chem 78:3706–3714. https://doi.org/10.1021/ac052020v

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Zimmermann S, Barth S, Baether WKM, Ringer J (2008) Miniaturized low-cost ion mobility spectrometer for fast detection of chemical warfare agents. Anal Chem 80:6671–6676. https://doi.org/10.1021/ac800559h

    Article  CAS  PubMed  Google Scholar 

  14. Oberreit D, Rawat VK, Larriba-Andaluz C et al (2015) Analysis of heterogeneous water vapor uptake by metal iodide cluster ions via differential mobility analysis-mass spectrometry. J Chem Phys 143:. https://doi.org/10.1063/1.4930278

  15. Dolan JW, Snyder LR, Djordjevic NM et al (1999) Reversed-phase liquid chromatographic separation of complex samples by optimizing temperature and gradient time: I. Peak capacity limitations. J Chromatogr A 857:1–20. https://doi.org/10.1016/S0021-9673(99)00765-7

    Article  CAS  PubMed  Google Scholar 

  16. Tolmachev AV, Clowers BH, Belov ME, Smith RD (2009) Coulombic effects in ion mobility spectrometry. Anal Chem 81:4778–4787. https://doi.org/10.1021/ac900329x

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Morrison KA, Bendiak BK, Clowers BH (2018) Assessment of dimeric metal-glycan adducts via isotopic labeling and ion mobility-mass spectrometry. J Am Soc Mass Spectrom 29:1638–1649. https://doi.org/10.1007/s13361-018-1982-2

    Article  CAS  PubMed  Google Scholar 

  18. Waraksa E, Perycz U, Namieśnik J et al (2016) Dopants and gas modifiers in ion mobility spectrometry. TrAC Trends Anal Chem 82:237–249. https://doi.org/10.1016/j.trac.2016.06.009

    Article  CAS  Google Scholar 

  19. Haun J, Leonhardt J, Portner C et al (2013) Online and splitless NanoLC × CapillaryLC with quadrupole/time-of- flight mass spectrometric detection for comprehensive screening analysis of complex samples. Anal Chem 85:10083–10090. https://doi.org/10.1021/ac402002m

    Article  CAS  PubMed  Google Scholar 

  20. Stephan S, Jakob C, Hippler J, Schmitz OJ (2016) A novel four-dimensional analytical approach for analysis of complex samples. Anal Bioanal Chem 408:3751–3759. https://doi.org/10.1007/s00216-016-9460-9

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

The authors would like to thank for financial aid support the German Federal Ministry for Economic Affairs and Energy within the agenda for the promotion of industrial cooperative research and development (IGF) on the basis of a decision by the German Bundestag. The access was opened by the German Federation of Industrial Research Association—AiF—and its member organisation Environmental Technology in short- member organization Environmental Technology (IGF Project No. 18861N). Special thanks to the technological SME SEADM, Dr. Marcus Winkler from Waters Corporation and Dr. Michael Schlüsener from the Bundesanstalt für Gewässerkunde for the kind opportunity to perform the resolution measurements on their systems. Additional thanks for the scientific exchange to Dr. Michaela Wirtz, Dr. Stefan Zimmermann and Dr. Terence Hetzel.

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Authors and Affiliations

  1. Institut für Energie- und Umwelttechnik e. V., Bliersheimer Str. 58-60, 47229, Duisburg, Germany

    Tobias Werres & Thorsten Teutenberg

  2. Instrumental Analysis, Niederrhein University of Applied Science, Frankenring 20, 47798, Krefeld, Germany

    Tobias Werres & Martin Jäger

  3. CURRENTA GmbH & Co. OHG, Alte Heerstraße, 41538, Dormagen, Germany

    Juri Leonhardt

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  1. Tobias Werres
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  2. Juri Leonhardt
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  3. Martin Jäger
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  4. Thorsten Teutenberg
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Correspondence to Thorsten Teutenberg.

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Published in Chromatographia’s 50th Anniversary Commemorative Issue.

Published in the topical collection 24th International Symposium on Separation Sciences combined with 21st International Conference Analytical Methods and Human Health with guest editors Milan Hutta and Dušan Berek.

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Werres, T., Leonhardt, J., Jäger, M. et al. Critical Comparison of Liquid Chromatography Coupled to Mass Spectrometry and Three Different Ion Mobility Spectrometry Systems on Their Separation Capability for Small Isomeric Compounds. Chromatographia 82, 251–260 (2019). https://doi.org/10.1007/s10337-018-3640-z

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  • DOI: https://doi.org/10.1007/s10337-018-3640-z

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