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Using Superficially Porous Particles and Ultrahigh Pressure Liquid Chromatography in Pharmacopeial Monograph Modernization of Common Analgesics

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Abstract

The higher pressures and flow rates needed to increase throughput in ultrahigh pressure liquid chromatography (UHPLC) can lead to thermal broadening due to viscous friction. The use of superficially porous particles and still air thermal environments can help reduce this broadening, but this has not yet been studied for applied, isocratic methods. Here, the use of five columns with varying lengths, diameters, and particle sizes were investigated for increased analytical throughput of pharmacopeial monograph methods for two over-the-counter analgesic drugs. System suitability parameters (resolution and peak asymmetry) and temperature changes across the axial length of the column were monitored at conditions near column or system pressure limits. Results from these investigations indicated that a 2.1 × 50-mm column packed with 2.6 µm particles provides the best opportunity for increased throughput, which was demonstrated with a 20-s cycle time method for the separation of four compounds (two active pharmaceutical ingredients, one impurity, and one internal standard) while maintaining a baseline resolution of 1.5 between all peaks.

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Funding

Partial funding for this work was provided by Rowan University College of Science and Mathematics start-up funding and a summer undergraduate research fellowship to G.A.K.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, Rowan University, Glassboro, NJ, USA

    Glenn A. Kresge & James P. Grinias

  2. Thermo Fisher Scientific, Waltham, MA, USA

    Jenny-Marie T. Wong

  3. Thermo Fisher Scientific, Germering, Germany

    Mauro De Pra & Frank Steiner

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  1. Glenn A. Kresge
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  2. Jenny-Marie T. Wong
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Correspondence to James P. Grinias.

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G.A.K. and J.P.G. declare no conflicts of interest. J.-M.T.W., M.D.P., and F.S. are employed by Thermo Fisher Scientific, a company that manufactures some of the technology used in this study.

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Published in the topical collection Rising Stars in Separation Science, as part of Chromatographia’s 50th Anniversary Commemorative Issue.

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Kresge, G.A., Wong, JM.T., De Pra, M. et al. Using Superficially Porous Particles and Ultrahigh Pressure Liquid Chromatography in Pharmacopeial Monograph Modernization of Common Analgesics. Chromatographia 82, 465–475 (2019). https://doi.org/10.1007/s10337-018-3593-2

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