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Development of a paper-based lateral flow immunoassay for simultaneous detection of lipopolysaccharides of Salmonella serovars

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Abstract

Lateral flow type detection is becoming interesting not only in regions with a poor medical infrastructure but also for practitioners in day-to-day clinical work or for veterinary control in case of possible epidemics. In this work, we describe the first steps of development of a multi-channel strip with potential internal calibration of multiparametric and colorimetric lateral flow assays for the simultaneous detection of the lipopolysaccharides (LPS) of Salmonella typhimurium (S. typhimurium) and Salmonella enteritidis (S. enteritidis). We structured four channels in the nitrocellulose membrane with a Yb:KGW solid-state femtosecond laser (“cold” ablation process) to form distinct tracks of porous material and used gold nanoparticles for the labeling of the antibodies. In addition, calibration curves of the spot intensities of both serovars are presented, and it was shown that no cross reactivity between the different capture antibodies and LPS occurred. Finally, we detected LPS of both Salmonella serovars simultaneously. The color changes (spot intensities of the reaction zones) were evaluated using the open-source image-processing program ImageJ.

Multiparametric testing, strip A was tested with LPS S. enteritidis ( c=0.01 g/L) and LPS S.typhimurium ( c=0.0001 g/L), strip B with LPS S. enteritidis ( c=0.001 g/L) and LPS S. typhimurium ( c=0.001g/L) and strip C with LPS S. enteritidis (c=0.0001 g/L) and LPS S. typhimurium ( c=0.01 g/L), and read-out

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Acknowledgements

This work was funded by the Bundesministerium für Wirtschaft und Energie within the project “Papierbasierte Low Cost Sensorik—Von der Mikrofabrikation bis zur Evaluation”, IGF-Vorhaben, Nr. 18148N/3.

We thank our project partners Tobias Tiemerding and Dr. Albert Still from OFFIS, Oldenburg and Denise van Rossum from Sartorius, Göttingen for the cooperation.

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Authors and Affiliations

  1. Institute of Physical and Theoretical Chemistry (IPTC), Eberhard Karls Universität Tübingen, Auf der Morgenstelle 18, 72076, Tuebingen, Germany

    Florian Schenk, Patricia Weber, Julian Vogler & Günter Gauglitz

  2. Institute of Microtechnology, Technische Universität Braunschweig, Alte Salzdahlumer Str. 203, 38214, Braunschweig, Germany

    Lars Hecht & Andreas Dietzel

Authors
  1. Florian Schenk
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  2. Patricia Weber
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  3. Julian Vogler
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  4. Lars Hecht
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  5. Andreas Dietzel
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  6. Günter Gauglitz
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Corresponding author

Correspondence to Günter Gauglitz.

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The authors declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors

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Published in the topical collection celebrating ABCs 16th Anniversary.

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Schenk, F., Weber, P., Vogler, J. et al. Development of a paper-based lateral flow immunoassay for simultaneous detection of lipopolysaccharides of Salmonella serovars. Anal Bioanal Chem 410, 863–868 (2018). https://doi.org/10.1007/s00216-017-0643-9

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  • DOI: https://doi.org/10.1007/s00216-017-0643-9

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