Abstract
In a previous study, we found hypercomplementaemia in the sera of acute myeloid leukemia patients. In this study we show that the supernatants of mononuclear cells, derived from peripheral blood taken in the blastic phase, from patients with acute myeloid leukemia (CM-AML) increased thein vitro complement protein synthesis of HepG2 hepatocellular carcinoma cells. This effect of CM-AML was mediated by heat labile soluble factors and involved the synthesis of mRNA and protein. Inhibition experiments with anti-cytokine antibodies and immunoaffinity chromatography revealed that this effect of CM-AML is mostly mediated by IL-1 and IL-6.
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Abbreviations
- IL-1:
-
interleukin-l-beta
- IL-2:
-
intcrleukin-2
- IL-6:
-
Interleukin-6
- TNF:
-
tumor necrosis factor
- C2:
-
complement factor 2
- C3:
-
complement factor 3
- C4:
-
complement factor 4
- -INH:
-
1-esterase inhibitor
- AML:
-
acute myeloid leukemia
- CM-AML:
-
conditioned medium of mononuclear cells derived from the blood of a patient with AML
- CM-PBMNC:
-
conditioned medium of peripheral blood mononuclear cells derived from the blood of a healthy volunteer
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Schmidt, B., Válay, M., Nahajevszky, S. et al. Complement synthesis influencing factors produced by acute myeloid leukemia blast cells. Pathol. Oncol. Res. 1, 54–59 (1995). https://doi.org/10.1007/BF02893584
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DOI: https://doi.org/10.1007/BF02893584