Abstract
When margarine is fortified with all-trans vitamin A the assay result, whether obtained by colorimetry or spectrophotometry, should be the same as the biological potency. This paper reports the effects to be expected on each method of assay by some commercial sources of vitamin A that containcis-isomers. In most cases the biological potencies are less than the potencies predicted by physicochemical procedures. Methods are presented to calculate the extent of assay differences introduced by thesecis-isomers. Different commercial sources of vitamin A, in an amount required for 15,000 “biological” units, would give values ranging from 15,200 to 20,800 units by “blue-color” assay, from 15,100 to 19,000 units by uncorrected extinction coefficient, and from 15,000 to 18,000 units by the USP XVI assay.
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References
Morton, R. A., and A. L. Stubbs, Analyst,71, 348 (1946).
Brown, P. S., W. P. Blum, and M. H. Stern, Nature,184, 1377 (1959).
Lehman, R. W., J. M. Dieterle, W. T. Fisher, and S. R. Ames, J. Am. Pharm. Assoc., Sci. Ed.,49, 363 (1960).
De Ritter, Elmer, J. Pharm. Sci.50, 510 (1961).
Ames, S. R., W. J. Swanson, and P. L. Harris, J. Am. Chem. Soc.,77, 4134 (1955).
Ames, S. R., W. J. Swanson, and P. L. Harris,Ibid.,,77, 4136 (1955)
Murray, T. K., and J. A. Campbell, Can. J. Biochem. Physiol.39, 1687 (1961).
“Definitive Rules for the Nomenclature of Amino Acids, Steroids, Vitamins, and Carotenoids,” by the Commission on the Nomenclature of Biological Chemistry, International Union of Pure and Applied Chemistry, J. Am. Chem. Soc.,82, 5575 (1960).
Rice, E. E., E. Primm, and A. I. Coombes, J. Assoc. Offic. Agr. Chemists.,31, 621 (1948).
Luckmann, F. H., D. Melnick, and H. W. Vahlteich, JAOCS,29, 121 (1952).
Robeson, C. D., W. P. Blum, J. M. Dieterle, J. D. Cawley, and J. G. Baxter, J. Am. Chem. Soc.,77, 4120 (1955).
Friedman, Leo, J. Assoc. Offic. Agr. Chemists, 43, 6 (1960). Also-Official Methods of Analysis of the A.O.A.C., Ninth Edition, p. 649 (1960).
Pharmacopeia of the United States, Sixteenth Revision, October 1, 1960.
Part 45—Oleomargarine; Margarine; Definition and Standard of Identity, Food Drug Cosmetic Law Reports, 21 CFR 45.1, p. 1641.
Pharmacopeia of the United States, Thirteenth Revision, april, 1, 1947.
Robeson, C. D., and J. G. Baxter, J. Am. Chem. Soc.,69, 136 (1947).
Ames, S. R., and R. W. Lehman, J. Assoc. Offic. Agr. Chemists,43, 21 (1960).
Hubbard, R., R. I. Gregerman, and G. Wald, J. Gen. Physiol.,36, 415 (1952-1953).
Robeson, C. D., J. D. Cawley, L. Weisler, M. H. Stern, C. C. Eddinger, and A. J. Chechak, J. Am. Chem. Soc.,77, 411 (1955).
Robeson, C. D., W. P. Blum, J. M. Dieterle, J. D. Cawley, and J. G. Baxter, J. Am. Chem. Soc.,77, 4120 (1955).
Lambertsen, G., and O. R. Braekkan, edited by G. Popjak and E. Le Breton, 56 (Butterworth, London, 1956).
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Lehman, R.W. Interpretation of various assays for vitamin a in margarine. J Am Oil Chem Soc 39, A12–A32 (1962). https://doi.org/10.1007/BF02637669
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DOI: https://doi.org/10.1007/BF02637669