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On-line straight-phase liquid chromatography/plasmaspray tandem mass spectrometry of glycerolipids

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Lipids

Abstract

Class separation of common glycerolipids on a diol-phase liquid chromatography column with a gradient of the mixed solvents hexane/isopropanol/acetic acid/triethylamine is compatible with on-line plasmaspray ionization mass spectrometry. The positive ion mass spectra exhibit prominent diacylglycerol and monoacylglycerol derived fragments, which can be utilized for quantification. In the selected-ion monitoring mode, the detection limit for phospholipids is in the low nanogram range. The abundance of the diacylglycerol and monoacylglycerol fragments reflects the relative fatty acid composition of the phospholipid classes. Collision-induced decomposition of diacylglycerol fragments with argon as collision gas unambiguously reveals the chain length and degree of unsaturation of the esterified fatty acids in the native lipid.

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Abbreviations

CID:

collision induced decomposition

FAB:

fast atom bombardment

HPLC/MS:

high-performance liquid chromatography/mass spectrometry

MS:

msss spectrometry

PA:

phosphatidic acid

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

PG:

phosphatidylglycerol

PI:

phosphatidylinositol

PSP:

plasmaspray

TIC:

total ion current

TSP:

thermospray

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Authors and Affiliations

  1. Division of chemical Ecology, Lund University, Helgonavägen 5, S-223 62, Lund, Sweden

    Anders Valeur & Göran Odham

  2. Hafslund Nycomed Innovation AB, Ideon, S-205 12, Malmö, Sweden

    Peter Michelsen

Authors
  1. Anders Valeur
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  2. Peter Michelsen
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  3. Göran Odham
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Valeur, A., Michelsen, P. & Odham, G. On-line straight-phase liquid chromatography/plasmaspray tandem mass spectrometry of glycerolipids. Lipids 28, 255–259 (1993). https://doi.org/10.1007/BF02536649

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  • DOI: https://doi.org/10.1007/BF02536649

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