Abstract
Multiple shoots were obtained from single node explants of matureGmelina arborea Roxb. on MS medium supplemented with 6-benzyladenine (BA). Seven to nine shoots were formed whenin vitro-derived single node explants were subcultured on MS medium supplemented with 1.1 μM BA. For root initiation the cut ends of microshoots were pulsed for 5 min with 246 μM indole-3-butyric acid and transferred to a plastic cup containing sterile vermiculite. The shoots were covered with polyethylene bag and maintained in a culture room. After hardening, plantlets were transferred to earthen pots containing a mixture of garden soil: compost and have been established in the field.
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Abbreviations
- BA:
-
6-benzyladenine
- IBA:
-
indole-3-butyric acid
- NAA:
-
1-napthaleneacetic acid
- IAA:
-
indole-3-acetic acid
- 2,4-d :
-
2,4-dichlorophenoxyacetic acid
References
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SharmaKK & ThorpeTA (1990)In vitro propagation of mulbery (Morus alba L.) through nodal segments. Sci. Hortic: 42. 307–320
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Kannan, V.R., Jasrai, Y.T. Micropropagation ofGmelina arborea . Plant Cell Tiss Organ Cult 46, 269–271 (1996). https://doi.org/10.1007/BF02307105
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DOI: https://doi.org/10.1007/BF02307105