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A screening method for isolating DNA repair-deficient mutants of CHO cells

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Somatic Cell Genetics

Abstract

A simple procedure for isolating mutagen-sensitive clones of CHO cells was developed and applied in mutant hunts in which colonies were screened for hypersensitivity to killing by ultraviolet radiation (UV), ethyl methanesulfonate (EMS), or mitomycin C (MMC). Each of two UV-sensitive clones studied in detail had a D37 dose of 1.0 J/m2 compared to 7.0 J/m2 for the wild-type cells, and each was shown to have no detectable repair replication following exposure to UV doses of up to 26 J/m2. Although these mutants resemble xeroderma pigmentosum human mutants with respect to their repair defect and cross-sensitivity to the carcinogen 4-nitroquinoline-1-oxide, one of two clones (UV-20) is characterized by extreme hypersensitivity to MMC (80-fold as compared to the wild type). Clones having hypersensitivity to alkylating agents, but not UV, were obtained using MMC and EMS. In the latter case the two clones had significantly increased sensitivity to the killing action of60Co γ-rays.

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Authors and Affiliations

  1. Biomedical Sciences Division, L-452, University of California, Lawrence Livermore Laboratory, 94550, Livermore, California

    Larry H. Thompson & Kerry Brookman

  2. Physics Division, Ontario Cancer Institute, M4X 1K9, Toronto, Ontario

    Jaime S. Rubin & Gordon F. Whitmore

  3. Laboratory of Radiobiology, University of California at San Francisco, 94143, San Francisco, California

    James E. Cleaver

Authors
  1. Larry H. Thompson
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  2. Jaime S. Rubin
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  3. James E. Cleaver
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  4. Gordon F. Whitmore
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  5. Kerry Brookman
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Notice: This report was prepared as an account of work sponsored by the United States Government. Neither the United States nor the United States Department of Energy, nor any of their employees, nor any of their contractors, subcontractors, or their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product or process disclosed, or represents that its use would not infringe privately owned rights.

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Thompson, L.H., Rubin, J.S., Cleaver, J.E. et al. A screening method for isolating DNA repair-deficient mutants of CHO cells. Somat Cell Mol Genet 6, 391–405 (1980). https://doi.org/10.1007/BF01542791

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  • DOI: https://doi.org/10.1007/BF01542791

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