Abstract
The electrophoretic mobilities of the 25S and 18S rRNAs of cotton seeds in polyacrylamide gel have been studied. A pyrimidyl-RNase hydrolysate of the high-molecular-weight rRNAs was separated into isopleths containing up to decanucleotides. The mono-, di-, and trinucleotide isopleths were separated, respectively, into CP and Up; ApCp, GpCp, ApUp, and GpUp; ApApCp, GpApCp, ApGpCp, ApApUp, GpGpCp, ApGpUp + GpApUp, and GpGpUp on a KhZh 1305 microcolumn liquid chromatograph.
Similar content being viewed by others
Literature cited
V. L. Leitin and M. I. Lerman, Biokhimiya,34, 839 (1969).
M. S. Kaulenas, Anal. Biochem.,41, 126 (1971).
Ts. V. Grancharova and T. A. Getova, Phytochemistry,15, 1431 (1976).
A. M. Érmatov, R. N. Nuriddinov, Khim. Prir. Soedin., 852 (1977).
U. E. Loening, Biochem. J.,102, 251 (1967).
U. E. Loening, Biochem. J.,113, 131 (1969).
U. E. Loening and J. Ingle, Nature (London),215, 363 (1967).
R. I. Mans and C. C. Alvarez, Anal. Biochem.,41, 560 (1971).
J. Huppert and J. Pelmont, Arch. Biochem. Biophys.,98, 214 (1962).
R. L. Villems and A. Ya. Lind, Uch. Zap. Tartussk. Univ.,285, 78 (1971).
M. A. Grachev, in: The Ultramicroanalysis of Nucleic Acids [in Russian], Moscow (1973), p. 104.
V. V. Vlasov, A. T. Puzyrev, Zh. P. Ébel', and R. Zhizhe, Bioorg. Khim.,7, 1487 (1981).
V. V. Vlasov, N. V. Melamed, V. E. Chizhikov, and M. A. Tukalo, Bioorg. Khim.,2, 892 (1976).
Kh. R. Nuriddinov, M. R. Nuriddinova, and R. N. Nuriddinov, Khim. Prir. Soedin., 110 (1981).
V. V. Vlesov and D. G. Knorre, Mol. Biol.,8, 234 (1974).
N. K. Kochetkov, É. I. Budovskii, E. D. Sverdlov, N. A. Simukova, M. F. Turchinskii, and V. N. Shibaev, The Organic Chemistry of the Nucleic Acids [in Russian], Moscow (1970), p. 177.
T. V. Venkstern and A. A. Baev, The Absorption Spectra of the Minor Components and Some Oligonucleotides of Ribonucleic Acids [in Russian], Moscow (1967), p. 38.
A. C. Peacock and C. W. Dingman, Biochemistry,6, 1818 (1967).
U. L. Raj Bhandary, R. D. Faulkner, and A. Stuart, J. Biol. Chem.,243, 575 (1968).
Additional information
Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 746–750, November–December, 1982.
Rights and permissions
About this article
Cite this article
Nuriddinov, K.R., Nuriddinova, M.R. & Nuriddinov, P.N. High-molecular-weight rRNAs of cotton seeds. Chem Nat Compd 18, 709–712 (1982). https://doi.org/10.1007/BF00579429
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF00579429