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Localization of nitrogenase in vesicles of Frankia sp. Cc1.17 by immunogoldlabelling on ultrathin cryosections

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Abstract

Immunogoldlabelling on ultrathin cryosections of Frankia sp. Cc1.17 showed specific labelling of nitrogenase in the spherical cells called vesicles. No label was found in the hyphae in any cells grown on a medium with combined nitrogen, nor in those to which no specific antiserum was added. Similar results were obtained with cultures grown under high (20%) and low (2%) oxygen tension in the gas phase.

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Abbreviations

BSA:

Bovine serum albumin fraction V (Sigma)

PBS:

phosphate buffered saline. Phosphate buffer 0.1 M, 8 g NaCl/1, 0.2 g KCl/1

PIPES:

Piperazine-1,4-bis(ethane sulphonic acid)

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Authors and Affiliations

  1. Department of Microbiology, Agricultural University, Hesselink van Suchtelenweg 4, NL-6703 CT, Wageningen, The Netherlands

    T. M. Meesters

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  1. T. M. Meesters
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Meesters, T.M. Localization of nitrogenase in vesicles of Frankia sp. Cc1.17 by immunogoldlabelling on ultrathin cryosections. Arch. Microbiol. 146, 327–331 (1987). https://doi.org/10.1007/BF00410930

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  • DOI: https://doi.org/10.1007/BF00410930

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