Abstract
Leaf tissue from five sugarcane clones with distinctive markers was cultured on a medium favoring callus growth. Transferred to a differentiation medium, calli produced over 5000 plants. Plants differentiated from two clones with stem markers exhibited a high rate of remission of the marker, but the marker reappeared in the vegetative progeny of these plants, and remission was, therefore, transient. Plants differentiated from callus from two clones with leaf markers showed a low rate of remission (2 or 3 per thousand) of the marker and the vegetative progeny was stable. A clone with variegated leaves produced plants with the majority having green leaves, some were albino, and some variegated, suggesting that plant differentiation may start with more than one cell. Permanent phenotypic change may result from tissue culture, but the results suggest that such changes are not frequent and may be confounded by temporary alterations or by chimeras formed in the process of differentiation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Breaux RD (1975) Radiosensitivity and selection for mosaic-resistant variants in sugarcane. Proc. Amer. Soc. Sugar Cane Technol. 4(NS):97–100
Chaleff RS (1983) Isolation of agronomically useful mutants from plant cell cultures. Science 219:676–682
Deerr N (1921) Cane Sugar. London: Norman Rodger, p. 61
Heinz DJ, Mee GP (1971) Morphologic, cytogenetic, and enzymatic variation in Saccharum species and hybrid clones, derived from callus tissue. Am J Bot 58: 257–262
Heinz DJ, Krishnamurthi M, Nickell LG, Maretzki A (1977) Cell, tissue and organ culture in sugarcane improvement. In: Reinert J, Bajaj YPS (eds) Plant cell, tissue and organ culture. Berlin: Springer-Verlag, pp. 3–17
Hughes CG (1964) Genetical effects. In: Hughes CG, Abbott EV, Wismer CA (eds) Sugar-Cane diseases of the World. Amsterdam: Elsevier, pp 265–296
Larkin PJ, Snowcroft WR (1981) Somoclonal variation — a novel source of variability from cell cultures for plant improvement. Theo and Appl Gen 60: 197–214
Liu MC, Chen WH (1976) Tissue and cell culture as aids to sugarcane breeding. 1. Creation of genetic variation through callus culture. Euphytica 25:393–403
Prased SC, Hassall DC, Krishnamurthi M (1983) Success rates of sub-cloning by tissue culture in selected hybrid varieties of sugarcane (Saccharum officinarum L.). Proc Int Soc Sugar Cane Technol 18:75–92 (bound preprint)
Secor GA, Shepard JF (1981) Variability of protoplast derived potato clones. Crop Sci 21:102–105
Thielke C (1970) Principles of chimeral variegation in grasses. Int Soc Sugar Cane Technol Path Newsletter 5:49–52
Vasil IK, Vasil V, Lu CY, Ozias-Akins P, Haydu Z, Wang DY (1983) Somatic embryogenesis in cereals and grasses. In: Earle ED, Demarly Y (eds) Variability in plants regenerated from tissue culture. New York: Praeger pp 3–21
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Irvine, J.E. The frequency of marker changes in sugarcane plants regenerated from callus culture. Plant Cell Tiss Organ Cult 3, 201–209 (1984). https://doi.org/10.1007/BF00040338
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00040338