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In vitro Plant Breeding towards Novel Agronomic Traits pp 43–58Cite as

Micropropagation of Enicostemma littorale Blume

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Abstract

An efficient in vitro plant regeneration protocol was established as a high-frequency in vitro mass propagation system for Enicostemma littorale Blume, an important diabetic medicinal plant. Decontaminated shoot tip and nodal segments (2 cm in length) were established on MS medium supplemented with different concentrations (0.5–2.5 mg/L) of 6-benzyladenine (BA), kinetin (Kin), and thidiazuron (TDZ) alone and in combination with different concentrations (0.3–1.8 mg/L) of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), and α-naphthalene acetic acid (NAA). The highest frequency (90%) of shoot regeneration and number of multiple shoots (179 shoots per explant) were achieved with a combination of Kin (1.5 mg/L) and NAA (0.6 mg/L). The shoot length (14.3 cm in length) was the best on the GA3 (1.2 mg/L) medium. Elongated shoots were rooted with different concentrations (0.2–1.0 mg/L) of α-naphthalene acetic acid (NAA), indole-3-acetic acid (IAA), and indole-3-butyric acid (IBA) alone. The maximum number of roots (17.4 roots per shoot) with a mean root length (15.2 cm in length) was obtained with MS medium containing IBA (0.4 mg/L). Plantlets were established in vermiculite, sand, and soil (1:1:2 w/v) mixture and acclimatized successfully in plant growth chamber. The acclimatized plants were established successfully in the field during winter season (19–25 °C), and the survival rate was 90%.

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Author information

Authors and Affiliations

  1. Department of Botany, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India

    M. Vigneswaran & N. Jayabalan

Authors
  1. M. Vigneswaran
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  2. N. Jayabalan
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Editors and Affiliations

  1. Department of Life Sciences, Central University of Jharkhand, Ranchi, Jharkhand, India

    Manoj Kumar

  2. Department of Plant Sciences, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, India

    Annamalai Muthusamy

  3. Himalayan School of Biosciences, Swami Rama Himalayan University Jolly Grant, Dehradun, Uttarakhand, India

    Vivek Kumar

  4. School of Life Sciences, Jawaharlal Nehru University, New Delhi, Delhi, India

    Neera Bhalla-Sarin

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Vigneswaran, M., Jayabalan, N. (2019). Micropropagation of Enicostemma littorale Blume. In: Kumar, M., Muthusamy, A., Kumar, V., Bhalla-Sarin, N. (eds) In vitro Plant Breeding towards Novel Agronomic Traits. Springer, Singapore. https://doi.org/10.1007/978-981-32-9824-8_3

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