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Sandwich Assays Based on QCM, SPR, Microcantilever, and SERS Techniques for Nucleic Acid Detection

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Biosensors Based on Sandwich Assays

Abstract

Signal transducers which can read the signal toward targets are widely used for nucleic acid assay. Typically, the signal transducers based on quartz crystal microbalance (QCM), surface plasmon resonance (SPR) sensor, microcantilever, and surface-enhanced Raman scattering (SERS) play a significant role in the development of techniques for the detection of nucleic acid. The combination of these techniques with sandwich assay has received extensive attention due to the advantages of sensitivity and specificity. In this chapter, we summarized the recent development of the nucleic acid sandwich assay based on QCM, SPR sensor, microcantilever, and SERS. Additionally, the advantages and disadvantages of these sandwich assays along with the challenges and prospects are also presented, devoting to guide researches to design more of robust sandwich assays for nucleic acid assay.

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Authors and Affiliations

  1. College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, People’s Republic of China

    Xiaoxia Hu & Quan Yuan

Authors
  1. Xiaoxia Hu
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  2. Quan Yuan
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Correspondence to Quan Yuan .

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Editors and Affiliations

  1. Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan, Hubei, China

    Fan Xia

  2. Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan, Hubei, China

    Xiaojin Zhang

  3. Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan, Hubei, China

    Xiaoding Lou

  4. College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, China

    Quan Yuan

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Hu, X., Yuan, Q. (2018). Sandwich Assays Based on QCM, SPR, Microcantilever, and SERS Techniques for Nucleic Acid Detection. In: Xia, F., Zhang, X., Lou, X., Yuan, Q. (eds) Biosensors Based on Sandwich Assays. Springer, Singapore. https://doi.org/10.1007/978-981-10-7835-4_9

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