Abstract
Pancreatic cancer is one of the most lethal cancers. Overexpression of epidermal growth factor receptor (EGFR) on the cell surface significantly enhances the tumor resistance, which makes the treatment even more challenging. Curcumin, a polyphenol found in turmeric (Curcumin longa), has been demonstrated to inhibit the growth, metastasis, and invasion of cancer cells via the EGFR signaling pathways. Our purpose is to develop an epidermal growth factor (EGF)-conjugated liposome-encapsulated curcumin (EGF-LP-Cur) to target the EGFR. As a result, the prepared liposomal EGF-LP-Cur had spherical vesicles with diameters of around 120 nm and contained high concentrations of curcumin and controlled concentrations of EGF. It was demonstrated to be stable in term of size and zeta potential within 21 days. The targeted formulation produced a significant increase of cytotoxicity on EGFR-overexpressed human pancreatic cancer cells as compared to the non-targeted one (p < 0.05). This is a promising candidate for the drug development in the treatment of pancreatic cancer, which remains challenging.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Chainani-Wu N (2003) Safety and anti-inflammatory activity of curcumin: a component of tumeric (Curcuma longa). J Altern Complement Med 9(1):161–168
Ruby AJ et al (1995) Anti-tumour and antioxidant activity of natural curcuminoids. Cancer Lett 94(1):79–83
Ramsewak RS, DeWitt DL, Nair MG (2000) Cytotoxicity, antioxidant and anti-inflammatory activities of curcumins I–III from Curcuma longa. Phytomedicine Int J Phytotherapy Phytopharmacology 7(4):303–308
Dhandapani KM, Mahesh VB, Brann DW (2007) Curcumin suppresses growth and chemoresistance of human glioblastoma cells via AP-1 and NFkappaB transcription factors. J Neurochem 102(2):522–538
Punfa W et al (2012) Enhancement of cellular uptake and cytotoxicity of curcumin-loaded PLGA nanoparticles by conjugation with anti-P-glycoprotein in drug resistance cancer cells. Acta Pharmacol Sin 33(6):823–831
Zhang L et al (2012) A novel folate-modified self-microemulsifying drug delivery system of curcumin for colon targeting. Int J Nanomed 7:151–162
Lu Y et al (2012) Preparation and in vitro evaluation of a folate-linked liposomal curcumin formulation. J Liposome Res 22(2):110–119
Manju S, Sreenivasan K (2012) Gold nanoparticles generated and stabilized by water soluble curcumin-polymer conjugate: blood compatibility evaluation and targeted drug delivery onto cancer cells. J Colloid Interface Sci 368(1):144–151
LeMaistre CF et al (1994) Targeting the EGF receptor in breast cancer treatment. Breast Cancer Res Treat 32(1):97–103
Rikimaru K et al (1992) Gene amplification and overexpression of epidermal growth factor receptor in squamous cell carcinoma of the head and neck. Head Neck 14(1):8–13
Saxby AJ et al (2005) Assessment of HER-2 status in pancreatic adenocarcinoma: correlation of immunohistochemistry, quantitative real-time RT-PCR, and FISH with aneuploidy and survival. Am J Surg Pathol 29(9):1125–1134
Moore MJ et al (2007) Erlotinib plus gemcitabine compared with gemcitabine alone in patients with advanced pancreatic cancer: a phase III trial of the National Cancer Institute of Canada Clinical Trials Group. J Clin Oncol Off J Am Soc Clin Oncol 25(15):1960–1966
Le UM, Cui Z (2006) Long-circulating gadolinium-encapsulated liposomes for potential application in tumor neutron capture therapy. Int J Pharm 312(1–2):105–112
Beduneau A et al (2007) Design of targeted lipid nanocapsules by conjugation of whole antibodies and antibody Fab’ fragments. Biomaterials 28(33):4978–4990
Thangapazham RL et al (2008) Evaluation of a nanotechnology-based carrier for delivery of curcumin in prostate cancer cells. Int J Oncol 32(5):1119–1123
Bhalerao SS, Raje A (2003) Harshal, preparation, optimization, characterization, and stability studies of salicylic acid liposomes. Drug Dev Ind Pharm 29(4):451–467
Smith SM et al (2011) A simple protocol for using a LDH-based cytotoxicity assay to assess the effects of death and growth inhibition at the same time. PLoS ONE 6(11):e26908
Olszewski-Hamilton U, Hamilton G (2011) Dependence of relative expression of NTR1 and EGFR on cell density and extracellular pH in human pancreatic cancer cell lines. Cancers 3(1):182–197
Acknowledgements
This work was supported by Sullivan University College of Pharmacy’s grant (RG-I-PS_2012_04) to UML.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Nature Singapore Pte Ltd.
About this paper
Cite this paper
Le, U.M., Ngo, D., Nguyen, T.M., Nguyen, Q.T., Ton, J. (2018). Enhanced Selective Cytotoxicity in Pancreatic Cancer Cells Using EGF-Conjugated Liposome-Encapsulated Curcumin. In: Vo Van, T., Nguyen Le, T., Nguyen Duc, T. (eds) 6th International Conference on the Development of Biomedical Engineering in Vietnam (BME6) . BME 2017. IFMBE Proceedings, vol 63. Springer, Singapore. https://doi.org/10.1007/978-981-10-4361-1_36
Download citation
DOI: https://doi.org/10.1007/978-981-10-4361-1_36
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-10-4360-4
Online ISBN: 978-981-10-4361-1
eBook Packages: EngineeringEngineering (R0)