Skip to main content
SpringerLink
Log in

Fabrication and Integration of PDMS-Glass Based Microfluidic with Optical Absorbance Measurement Device for Coliform Bacteria Detection

  • Conference paper
  • First Online:
Book cover 6th International Conference on the Development of Biomedical Engineering in Vietnam (BME6) (BME 2017)

Part of the book series: IFMBE Proceedings ((IFMBE,volume 63))

Abstract

The detection of coliform bacteria which contain the disease-causing microorganism is a useful indication for water contamination. Currently, the emerging of technology in molecular biology research and industry is in demand for portable and miniaturized system. This paper demonstrates the development and integration of microfluidic and optical absorbance measurement device for portable coliform bacteria detection. The microfluidic device was fabricated with glass and polydimethylsiloxane (PDMS) material using photolithography, replica molding (soft lithography), and oxygen plasma bonding techniques. Then, the optical absorbance measurement device for coliform bacteria detection was developed using 470 nm blue light emitting diode (LED), photo detector, ARDUINO microcontroller, liquid crystal display (LCD), and mechanical elements. The coliform bacteria suspension sample was inserted into the microfluidic device and the presence of coliform bacteria was analyzed using the developed optical absorbance measurement device. The absorbance measurement from the prototype and colony number of the coliform bacteria samples were collected and analyzed. The final analysis had indicated that the developed prototype was able to detect the coliform bacteria in suspension at the lowest detection of 17,200 CFU/ml.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Noble RT, Moore DF, Leecaster MK, McGee CD, Weisberg SB (2003) Comparison of total coliform, fecal coliform, and enterococcus bacterial indicator response for ocean recreational water quality testing. Water Res 37(7):1637–1643

    Article  Google Scholar 

  2. Edberg S, Rice E, Karlin R, Allen M (2000) Escherichia coli: the best biological drinking water indicator for public health protection. J Appl Microbiol 88(1):106S–116S. Notari B (1993) Catalysis today 18:163

    Google Scholar 

  3. Kim J, Knapp D (2001) Miniaturized multichannel electrospray ionization emitters on poly(dimethylsiloxane) microfluidic devices. Electrophoresis 22(18):3993–3999

    Article  Google Scholar 

  4. Noble R, Weisberg R (2005) A review of technologies for rapid detection of bacteria in recreational waters. J Water Health 3:381–392

    Article  Google Scholar 

  5. Beer (1852) Bestimmung der Absorption des rothen Lichts in farbigen Flüssigkeiten (Determination of the absorption of red light in colored liquids). Ann Phys Chem 162(5):78–88

    Article  Google Scholar 

  6. Lambert J (2001) Photometry: or on the measure and gradations of light, colors, and shade 1760 (translated from the Latin by David L. DiLaura, vol 22)

    Google Scholar 

  7. Kubitschek H (1990) Cell volume increase in Escherichia coli after shifts to richer media. J Bacteriol 172(1):94–101

    Article  Google Scholar 

  8. Myers J, Curtis B, Curtis W (2013) Improving accuracy of cell and chromophore concentration measurements using optical density. BMC Biophys 6(1):4

    Article  Google Scholar 

  9. Krueger AP (1930) A method for the quantitative estimation of bacteria in suspensions. J Gen Physiol 13(5):553–556

    Article  Google Scholar 

  10. Guerrero M, Jones R (1997) Light-induced absorbance changes associated with photo inhibition and pigments in nitrifying bacteria. Aquat Microb Ecol 13:233–239

    Article  Google Scholar 

  11. Sharpe M (1984) Stray light in UV-VIS spectrophotometers. Anal Chem 56(2):339A–356A

    Google Scholar 

  12. Fuwa K, Valle B (1963) The physical basis of analytical atomic absorption spectrometry: the pertinence of the Beer-Lambert law. Anal Chem 35(8):942–946

    Article  Google Scholar 

  13. Putman M, Burton R, Nahm M (2005) Simplified method to automatically count bacterial colony forming unit. J Immunol Methods 302(1–2):99–102

    Article  Google Scholar 

  14. Whitlock J, Jones D, Harwood V (2002) Identification of the sources of fecal coliforms in an urban watershed using antibiotic resistance analysis. Water Res 36(17):4273–4282

    Article  Google Scholar 

  15. Held G (2009) Introduction to Light emitting diode technology and applications. CRC Press, Boca Raton, pp 137–138

    Google Scholar 

  16. Boyes W (2003) Instrumentation Reference Book. Butterworth-Heinemann, Boston, pp 501–502

    Google Scholar 

Download references

Acknowledgements

The authors would like to thank Ministry of Higher Education Malaysia for the financial support through PRGS grant Vote No. G005. We also acknowledged Universiti Tun Hussein Onn Malaysia (UTHM) for technical support.

Author information

Authors and Affiliations

  1. Microelectronics and Nanotechnology–Shamsuddin Research Centre (MiNT-SRC), Universiti Tun Hussein Onn Malaysia, 86400, Batu Pahat, Johor, Malaysia

    N. M. Salih, M. Z. Sahdan, M. Morsin & M. T. Asmah

Authors
  1. N. M. Salih
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Z. Sahdan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Morsin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. T. Asmah
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. Z. Sahdan .

Editor information

Editors and Affiliations

  1. Biomedical Engineering Department, Vietnam National University, Ho Chi Minh , Ho Chi Minh City, Vietnam

    Toi Vo Van

  2. Department of Biomedical Engineering, Vietnam National University, Ho Chi Minh Department of Biomedical Engineering, Ho Chi Minh City, Vietnam

    Thanh An Nguyen Le

  3. Biomedical Engineering Department, Vietnam National University, Ho Chi Minh Biomedical Engineering Department, Ho Chi Minh City, Vietnam

    Thang Nguyen Duc

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer Nature Singapore Pte Ltd.

About this paper

Cite this paper

Salih, N.M., Sahdan, M.Z., Morsin, M., Asmah, M.T. (2018). Fabrication and Integration of PDMS-Glass Based Microfluidic with Optical Absorbance Measurement Device for Coliform Bacteria Detection. In: Vo Van, T., Nguyen Le, T., Nguyen Duc, T. (eds) 6th International Conference on the Development of Biomedical Engineering in Vietnam (BME6) . BME 2017. IFMBE Proceedings, vol 63. Springer, Singapore. https://doi.org/10.1007/978-981-10-4361-1_13

Download citation

  • DOI: https://doi.org/10.1007/978-981-10-4361-1_13

  • Published:

  • Publisher Name: Springer, Singapore

  • Print ISBN: 978-981-10-4360-4

  • Online ISBN: 978-981-10-4361-1

  • eBook Packages: EngineeringEngineering (R0)

Publish with us

Policies and ethics

Search

Navigation