Abstract
Granule-bound starch synthase (GBSS) is the primary enzyme responsible for the synthesis of amylose in amyloplasts of cereal endosperm cells. In bread wheat there are three structural genes (Wx-A1, Wx-B1, and Wx-D1) encoding for isoforms of GBSS. The loss of one or more GBSS isoforms results in the reduction (partial-waxy) or absence (waxy) of amylose in the starch. Waxy wheats may find application in the production of modified food starch and their flour may be used to extend the shelf life of baked products. In order to breed high quality wheats able to produce bread with delayed staling, the genetic variability for the waxy trait in our germplasm has been investigated. We analysed 288 cultivars of bread wheat, 139 cultivars of durum wheat and about 200 accessions from other Triticum species. Gel electrophoresis showed 63 bread wheats deficient in the Wx-B1, one in the Wx-A1 and one in the Wx-D1 protein isoforms, as well as one Triticum dicoccum lacking the Wx-A1 isoform. None of the analysed Triticum monococcum, Triticum durum, Triticum spelta and Triticum timopheevi accessions showed mutations at the Wx loci. The wheat accessions with Wx mutations were evaluated with a Rapid Visco Analyser (RVA) to investigate starch properties. All the analysed cultivars showed Peak Viscosity and Final Viscosity different from the normal wheat. Other analyses to evaluate the rheological characteristics of the partial-waxy genotypes are under way and a breeding programme to select new waxy wheat varieties is in progress
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References
Batey, I.L., Gras, P.W., Curtin, B.M. (1997): Contribution of the chemical structure of wheat starch to Japanese noodle quality. JScience of Food and Agric., 74, 503–508.
Biliaderis, C.G. (1992): Structures and phase transition of starch in food system. Food Technology, 46, 98–109.
Chao, S., Sharp, P.J., Worland, E.J., Warham, E.J. Koebner, R.M.D., Gale, M.D. (1989): RFLP-based genetic maps of wheat homoeologous group 7 chromosomes. Theor. Appl. Genet., 78, 495–505.
D’Appolonia, B.L., Gilles, K.A., Osman, E.M., Pomeranz, Y. (1971): Carbohydrates. In: Pomeranz, Y. (ed), Wheat - Chemistry and Technology, 2nd ed.. AACC, St. Paul, MN, 301-
Eliasson, A.C. (1985): Retrogradation of starch as measured by differential scanning colorimetry. In: Hill, R.D., Munk, L. (eds), New Approaches to Research on Cereal Carbohydrates. Elsevier Science, Amsterdam, 93-
Graybosch, R.A., Chung, O.K. (1998): Null alleles at the Wx loci: effects on starch pasting properties of hard winter wheats. In. Slinkard, A.E. (ed), Proc 9 th Int. Wheat Genet. Symp. Univ. Ext. Press. Univ. Saskatchewan, Vol. 4, 163–165.
Graybosch, R.A., Peterson, C.J., Hansen, L.E., Rahman, S., Hill, A. Skerrit, J.H. (1998). Identification and characterization of U.S. wheats carrying null alleles at wx loci. Cereal Chem., 75, 162–165.
Hayakawa, K., Tanaka, K., Nakamura, T., Endo, S., Hoshino, T. (1997): Quality characteristics of waxy hexaploid wheat (Triticum aestivum L.): properties of starch gelatinisation and retrogradation. Cereal Chem., 74, 576–580.
Hegstad, J.B., Kianian, S.F., McMullen, M.S., Doehlert, D.C. (1998): Development of waxy (low amylose) durum cultivars. In: Slinkard, A.E. (ed), Proc. 9 th Int. Wheat Genet Symp., Univ. Ext. Press., Univ. Saskatchewan, Vol. 4, 172–174.
Kiribuchi-Otobe, C., Nagamina, T., Yanagisawa, T., Ohnishi, M. Yamaguchi, I. (1997): Production of hexaploid wheats with waxy endosperm character. Cereal Chem, 74, 72–74.
Kiribuchi-Otobe, C., Yanagisawa, T., Yamaguchi, I., Yoshida, H. (1998): Wheat mutant with waxy starch showing stable hot pasta viscosity. Cereal Chem., 75, 671–672.
Landi, A. (1995): Durum wheat, semolina and pasta quality characteristics for an Italian food company. In: Di Fonzo, N., Kaan, F., Nachit, M. (eds), Durum wheat quality in the Mediterranean Region. CIHEAM, 33–42.
Miura, H., Tanii, S. (1994): Endosperm starch properties in several wheat cultivars preferred for Japanese noodles. Euphytica, 72, 171–175.
Nakamura, T., Yamamori, M., Hidaka, S., Hoshino, T. (1992): Expression of HMW Wx protein in common wheat (Triticum aestivum L.) cultivars. Jpn. J. Breed., 42, 681–685.
Nakamura, T., Yamamori, M., Hirano, H., Hidaka, S. (1993a): Identification of three Wx protein in wheat (Triticum aestivum L.). Biochem. Genet., 31, 75–86.
Nakamura, T., Yamamory, M., Hidaka, S., Hoshino, T. (1993b): Decrease of waxy (Wx) protein in two common wheat cultivars with low amylose content. Plant Breed., 111, 99–105.
Nakamura, T., Yamamory, M., Hirano, H., Hidaka, S., Nagamine, T. (1995): Production of waxy (amylose-free) wheats. Mol Gen Genet., 248, 253–259.
Preiss, J., Sivak, M.N. (1996): Starch synthesis in sink and sources. In: Zamski, E., Schaffer, A.A. (eds) Photoassimilate Distribution in Plants and Crops. Source-sink Relationships. Marcel Dekker, New York, 63–96.
Urbano, M., Colaprico, G., Margiotta, B. (1996): Waxy protein variation in tetraploid and hexaploid wheats. In: Wrigley, C.W. (ed), Proc. 6 1 “ Int. Gluten Workshop ”Gluten 96“, Melbourne, Australia, 64–67.
Yamamori, M., Nakamura, T., Endo, T.R., Nagamina, T. (1994): Waxy protein deficiency and chromosomal location of coding genes in common wheat. Theor. Appl Genet, 89, 179–184.
Yamamori, M, Nakamura, T., Nagamine, T. (1995): Polymorphism of two waxy proteins in the emmer group of tetraploid wheat, Triticum dicoccoides, T. dicoccum, and T. durum. Plant Breed., 114, 215–218.
Zeng, M., Morris, C.F., Batey, I.L., Wrighley C.W. (1997): Source of variation for starch gelatinisation, pasting, and gelation properties in wheat. Cereal Chem., 74, 63–71.
Zhao, X.C., Sharp. P.J. (1996): An improved 1-D SDS-PAGE method for the identification of three bread wheat `waxy’ protein. J. Cereal Sci., 23, 191–193.
Zhao, X.C., Batey, I.L., Sharp, P.J., Crosbie, G., Barclay, I., Wilson, R., Morell, M.K., Appels, R. (1998): A single genetic locus associated with starch granule properties and noodle quality in wheat. J. Cereal Sci., 27, 7–13.
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Boggini, G., Cattaneo, M., Paganoni, C., Vaccino, P. (2001). Genetic Variation for Waxy Proteins and Starch Properties in Italian Wheat Germplasm. In: Bedö, Z., Láng, L. (eds) Wheat in a Global Environment. Developments in Plant Breeding, vol 9. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-3674-9_30
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DOI: https://doi.org/10.1007/978-94-017-3674-9_30
Publisher Name: Springer, Dordrecht
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