Abstract
The fate of l4C-naringenin during its specific activation of nod genes in Rhizobium leguminosarum bv. viciae was examined. After incubation with either strain RBL5560 or its pSym-cured derivative in a medium supplemented with 14C-naringenin at nod gene-inducing concentrations of 2 nM (ca. 12.5 kBq) plus cold acetate (0.5 μM), a radiocarbon inventory for the cells and supernatant extracts was obtained. The level of 14C-label incorporation was also determined in the fractionated cellular components. Using 14C-acetate at 0.5 μM (1036 kBq) and cold naringenin (2 nM) in incubations with strain RBL5560 as a separate treatment, the Nod metabolites were detected by thin layer and high performance liquid Chromatographie methods and the data provided the basis for identification of the Nod factors from the supernatant obtained from 14C-naringenin treatments. Subsequent radio-biochemical and chemical analyses revealed that RBL5560 cells assimilated 14C-naringenin during the activation of nod genes. Our analysis also showed that labelled carbon atoms from the 14C-naringenin were incorporated into the acyl moiety of a lipo-oligosaccharide Nod factor, NodRlv IV, present in the culture supernatants of RBL5560. The pSym-cured derivative failed to synthesize any Nod metabolites in a 14C-naringenin supplemented medium. The tracing of flavonoid-derived carbon atoms to the acyl chain of a host-specific Nod factor, a moiety that defines host specificity for this Rhizobium, adds a new dimension to the signalling function of flavonoids in Xcgumc-Rhizobium interactions.
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Abbreviations
- Ac:
-
acyl chain
- ca:
-
calculated approximately
- dpm:
-
disintegrations per minute
- HPLC:
-
High Performance Liquid Chromatography
- pSym:
-
symbiotic plasmid
- R:
-
Rhizobium
- TLC:
-
Thin Layer Chromatography
References
De Cooman L, Everaert E S W, Fache P, Vandecasteele K and Van Suraere C F 1993 Flavonoid biosynthesis in petals of Rhododendron simsii. Phytochemistry 33, 1419–1426.
Gajendiran N and Mahadevan A 1988 Utilization of catechin by Rhizobium sp. Plant and Soil 108, 263–266.
Hanson R S and Phillips JA 1981 Chemical composition: Chapter IV: Metabolism. Ed. W A Wood, in Manual of Methods for General Bacteriology. Ed. P Gerhardt, pp 328–364. American Society for Microbiology, Washington, DC, USA.
Hubac C, Ferran J, Tremolieres A and Kondorosi A 1994 Luteolin uptake by Rhizobium meliloti: evidence for several steps including an active extrusion process. Microbiology 140, 2769–2774.
Perret X, Fellay R, Bjourson A J, Cooper J E, Brenner S and Broughton WJ 1994 Subtraction hybridization and shot-gun sequencing: a novel approach to identify symbiotic loci. Nucleic Acids Res. 22, 1335–1341.
Peters N K, Frost J W and Long S R 1986 A plant flavone, luteolin, induces expression of Rhizobium meliloti nodulation genes. Science 233, 977–980.
Peters N K and Verma D P S 1990 Phenolic compounds as regulators of gene expression in plant-microbe interactions. Mol. Plant-Microbe Interactions 3, 4–8.
Rao J R, Sharma N D, Hamilton J T G, Boyd D R and Cooper J E 1991 Biotransformation of the pentahydroxyflavone quercetin by Rhizobium loti and Brady rhizobium strains (Lotus). Appl. Environ. Microbiol. 57, 1563–1565.
Rao J R and Cooper J E 1994 Rhizobia catabolize nod gene inducing flavonoids via C-ring fission mechanisms. J. Bacteriol. 176, 5409–1565.
Rao J R and Cooper J E 1995 Soybean nodulating rhizobia modify nod gene inducers daidzein and genistein to yield aromatic products that can influence gene inducing activity. Mol. Plant-Microbe Interactions 8, 855–862.
Redmond J W, Batley M A, Djordjevic M A, Innés R W, Kuempel P L, and Rolfe B G 1986 Flavones induce expression of nodulation genes in Rhizobium. Nature 323, 632–635.
Roche P, Lerouge P, Ponthus C and Prome J-C 1991 Structural determination of bacterial nodulation factors involved in the Rhizobium meliloti- Alfalfa symbiosis. Biol. Chem. 266, 10933–10949.
Sadowsky M J, Olsen E R, Foster V E, Kosslak R M and Verma DPS 1988 Two host-inducible genes of Rhizobium fredii and characterization of the inducing compound. J. Bacteriol. 170, 171–178.
Spaink H P, Sheeley D M, Van Brussel A A N, Glushka J, York W S, Tak T, Geiger O, Kennedy E P, Reinhold V N and Lugten-berg B J J 1991 A novel highly unsaturated fatty acid moiety of lipo-oligosaccharide signals determines host specificity of Rhizobium. Nature 354, 125–130.
Spaink H P, Aarts A, Stacey G, Bloemberg G V, Lugtenberg B J J and Kennedy E P 1992 Detection and separation of Rhizobium and Brady rhizobium Nod metabolites using thin layer chromatogra-phy. Mol. Plant-Microbe Interactions 5, 72–80.
Spaink H P 1994 The molecular basis for the host specificity of the Rhizobium bacteria. Antonie van Leeuwenhoek 65, 81–98.
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Rao, J.R., Cooper, J.E., Everaert, E.S.W., De Cooman, L. (1996). Assimilation of nod gene inducer 14C-naringenin and the incorporation of labelled carbon atoms into the acyl side chain of a host-specific Nod factor produced by Rhizobium leguminosarum bv. viciae. In: Elkan, G.H., Upchurch, R.G. (eds) Current Issues in Symbiotic Nitrogen Fixation. Developments in Plant and Soil Sciences, vol 72. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5700-1_8
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DOI: https://doi.org/10.1007/978-94-011-5700-1_8
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