Abstract
For fruit taste improvement of temperate horticultural crops - apple, pear, strawberry and carrot the gene of supersweet protein thaumatin II from Thaumatococcus daniellii Benth. was used in our research. Based on the coding sequence, which have been cloned in Unilever, and pBI121 we constructed the binary vector for its expression in plants. The successful transformation of apple, carrot, pear and strawberry have been obtain by usage the disarmed supervirulent agrobacterial strain CBE21 received from “Bioengineering Center” RAS. The introduction of thaumatin gene was confirmed by PCR analysis. The high thaumatin II expression was observed in callus tissues and lower one in leaves of greenhouses plants of apple, pear and carrot roots.
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Dolgov, S.V., Lebedev, V.G., Firsov, A.P., Taran, S.A., Tjukavin, G.B. (1999). Expression of thaumatin II gene in horticultural crops. In: Mugnozza, G.T.S., Porceddu, E., Pagnotta, M.A. (eds) Genetics and Breeding for Crop Quality and Resistance. Developments in Plant Breeding, vol 8. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-4475-9_19
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DOI: https://doi.org/10.1007/978-94-011-4475-9_19
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