Abstract
Differential screening of cDNA libraries of mid-maturation stage zygotic embryo 4–6 day old seedlings yielded a set of cDNAs preferentially expressed dining embryogenesis and germination, respectively. Sequence analysis of embryo cDNAs identified these clones as ena isoforms of 2S storage proteins- albumins, and an MT-like protein. The expression of these ger different stages of zygotic and somatic embryogenesis was examined by Northern blot analysis different patterns of expression were observed for various isoforms of albumins. The MT-like gene expressed in embryos and needles and its expression was regulated by ABA, osmoticum as well as i ions. Partial DNA sequence analysis and predicted amino acid sequences of germination — assa cDNAs show we have isolated clones with homology to a range of plant proteins: including a chape 60-ß, low molecular weight heat shock proteins (LMW HSPs), a luminal binding protein (BiP), a ty chlorophyll a/b binding protein (CAB), a thiol-protease precursor and a NADPH-P450 reductase. I seeds all the clones detected transcripts which increased by 2 days of germination.
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References
Bewley, J.D. and Marcus, A. (1990) Gene expression in seed development and germination, Prog. Nuc. Acids Res. Mol. Biol. 38, 165–193.
Denecke, J., Goldman, M.H.S., Demolder, J., Seurinck, J. and Botterman, J. (1991) The tobacco luminal binding protein is encoded by a multigene family, Plant Cell 3, 1025–1035.
Gupta, P.K. and Grob, J.A. (1995) Somatic embryogenesis in conifers, in S. Jain, P. Gupta and R. Newton (eds.), Somatic Embryogenesis in Woody Plants, Kluwer Academic Publishers, Dordrecht, Vol l, pp. 81–89.
Hemmingsen, S.M., Woolford, C., van der Vies, S.M., Tilly, K. and Dennis, D.T. (1988) Homologous plant and bacterial proteins chaperone oligomeric protein assembly, Nature 333, 330–334.
Jakob, U., Gaestel, M., Engel, K. and Buchner, J. (1993) Small heat shock proteins are molecular chaperones, J. Biol. Chem. 268, 1517–1520.
Kaukinen, K., Tranbarger, TJ. and Misra, S. (in press) Post-germination-induced and hormonallydependent expression of low molecular weight heat shock protein genes in Douglas fir, Plant Mol. Biol.
Kojima, K., Yamamoto, N. and Sasaki, S. (1992) Structure of the pine (Pinus thunbergii ) chlorophyll a/b-binding protein gene expressed in the absence of light, Plant Mol. Biol. 19, 405–410.
Koizumi, M., Yamaguchi-Shinozaki, K., Tsuji, H. and Shinozaki, K. (1993) Structure and expression of two genes that encode distinct drought-inducible cysteine proteinases in Arabidopsis thaliana, Gene 129, 175–182.
Krebbers, E., L. Herdies, De Clerco, A., Seurink, J., Leemans, J., Van Damme, J., Segura, M., Gheysen, G., Van Montagu, M. and Vandekerckhove, J. (1988) Determination of the processing sites of an Arabidopsis 2S albumin and characterization of the complete gene family, Plant Physiol. 87, 859–866.
Lane, B.G. (1991) Cellular desiccation and hydration: developmentally regulated proteins, and the maturation and germination of seed embryos, FASEB J. 5, 2893–2901.
Leal, I. and Misra, S. (1993) Molecular cloning and characterization of a legumin-like storage protein cDNA of Douglas-fir seeds, Plant Mol. Biol. 21, 709–715.
Ledger, S.E. and Gardner, R.C. (1994) Cloning and characterization of five cDNAs for genes differentially expressed during fruit development of kiwifruit (Actinidia delicosa var. deliciosa), Plant Mol. Biol. 25, 877–886.
Lu, A.Y.H., Junk, K.W. and Coon, MJ. (1969) Resolution of the cytochrome P-450-containing ω-hydroxylation system of liver microsomes into three components, J. Biol. Chem. 244, 3714–3721.
Mansfield, M.A. and Key, J.L. (1987) Synthesis of the low molecular weight heat shock proteins in plants, Plant Physiol. 84, 1007–1017.
Misra, S. (1994) Conifer zygotic embryogenesis, somatic embryogenesis, and seed germination: biochemical and molecular advances, Seed Sci. Res. 4, 357–384.
Misra, S. and Green, M. (1994) Legumin-like storage polypeptides of conifer seeds and their antigenic cross-reactivity with US globulins from angiosperms, J. Exp. Bot. 45, 269–274.
Owens, J.N., Morris, S J. and Misra, S. (1993) The ultrastructural, histochemical and biochemical development of the post-fertilization megagametophyte and the zygotic embryo of Pseudotsuga menziesii (Mirb.) Franco, Can. J. For. Res. 23, 816–827.
Shet, M.S., Sathasivan, K., Arlotto, M.A. and Mehdy, M.C. (1993) Purification, characterization, and cDNA cloning of an NADPH-cytochrome P450 reductase from mung bean, Proc. Natl. Acad. Sci. USA 90, 2890–2894.
Tseng, T.S., Yeh, K.W., Yeh, C.H., Cheng, F.C., Chen, Y.M. and Lin, C.Y. (1992) Two rice (Oryza saliva ) full length cDNA clones encoding low molecular weight heat shock proteins, Plant Mol. Biol 18, 963–965
Taylor, M.A., Davies, H.V., Smith, S.B., Abruzzese, A. and Gosling, P.G. (1993) Cold-induced changes in gene expression during dormancy breakage in seeds of Douglas-fir (Pseudotsuga menziesii),J. Plant Physiol. 142, 120–123.
Tranbarger, TJ. and Misra, S. (in press) Molecular characterization of a set of cDNAs differentially expressed during germination and early seedling growth, Physiol. Plant.
Vierling, E. (in press) Structure and in vitro molecular chaperone activity of cytosolic small heat shock proteins from pea, J. Biol. Chem.
Zabeleta, E., Oropeza, A., Jimenez, B., Salerno, G., Crespi, M. and Herrera-Estrella, L. (1992) Isolation and characterization of genes encoding chaperonin 60ß from Arabidopsis thaliana, Gene 111, 175–181.
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Misra, S., Chatthai, M., Tranbarger, T.J., Forward, B.S., Kaukinen, K.H. (1996). Differentially Regulated Gene Sets in Douglas Fir Seeds and Somatic Embryos.. In: Ahuja, M.R., Boerjan, W., Neale, D.B. (eds) Somatic Cell Genetics and Molecular Genetics of Trees. Forestry Sciences, vol 49. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3983-0_27
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DOI: https://doi.org/10.1007/978-94-011-3983-0_27
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