Abstract
Genetic transformation has been used to improve poplar tolerance toward Chiysomela tremulae, a coleoptera causing severe damages in nurseries and young poplar plantations. We have shown in an in vitro assay that cysteine proteinase represent the major proteinase activity in the midgut of C. tremulae. Moreover, in this system the cysteine proteinase inhibitor OCI effectively inhibits most of the digestive proteinase activity. This proteinase inhibitor and the Bacillus thuringiensis δ-endotoxin CRY IIIA, also known to be active against coleoptera, were both evaluated for their toxicity against C. tremulae. Transgenic poplars expressing either ocI or cry IIIA gene were produced. Insects feeding on this transgenic poplars exhibit reduced larval growth, altered development and increased mortality when compared to the control.
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© 1996 Springer Science+Business Media Dordrecht
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Cornu, D. et al. (1996). Expression of A Proteinase Inhibitor and A Bacillus Thuringiensis δ-Endotoxin in Transgenic Poplars.. In: Ahuja, M.R., Boerjan, W., Neale, D.B. (eds) Somatic Cell Genetics and Molecular Genetics of Trees. Forestry Sciences, vol 49. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3983-0_18
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DOI: https://doi.org/10.1007/978-94-011-3983-0_18
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