Abstract
A cDNA clone coding for human IL-3 was the starting point for the clinical development of this protein. The coding information for this multi-colony stimulating factor was expressed in a wide range of host organisms using the Gist-brocades PLUGBUG (TM) concept. After careful evaluation of the advantages and disadvantages of each system Bacillus licheniformis licheniformis was chosen as the production organism. A fermentation and downstream processing procedure was developed enabling the scale-up to desired volumes and quantities. The purified and formulated product entered Phase I clinical trials in November 1989.
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van Leen, R.W., Lemson, P.J., Bakhuis, J.G. (1990). Heterologous Expression of Human Interleukin-3. In: Crommelin, D.J.A., Schellekens, H. (eds) From Clone to Clinic. Developments in Biotherapy, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3780-5_32
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DOI: https://doi.org/10.1007/978-94-011-3780-5_32
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