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Analysis of tissues for EcR and RXR nuclear receptor gene expression during vitellogenesis in the soft tick Ornithodoros moubata

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Trends in Acarology

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Abstract

Vitellogenin (Vg) synthesis and oocyte development appear to be induced by ecdysteroids in the soft tick Ornithodoros moubata. Vg transcription in insects has been shown to be mediated by two nuclear receptors, an ecdysteroid receptor (EcR) and a retinoid X receptor (RXR). EcR and RXR expression in female ticks increase after engorgement. However, there is little information about EcR and RXR expression in tissues as related to reproduction. Therefore, the sites of EcR and RXR expression during vitellogenesis were determined. Expression levels of EcR and RXR were determined for salivary glands, midgut, ovary, anterior reproductive tract, fat body, muscle, and cuticle of mated females by reverse transcriptase RT-PCR and real-time PCR. Ovaries showed constant expression of EcR and RXR throughout the adult female stage. The fat body showed higher expression of EcR and RXR on one day after engorgement. The fat body of female ticks has been reported to be the organ of Vg synthesis. Therefore, increases in EcR and RXR of the fat body support the hypotheses that the site of Vg synthesis is the fat body and ecdysteroids regulate this synthesis in O. moubata. EcR and RXR in the ovary and anterior reproductive tract may also play roles in the regulation of oocyte development.

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References

  • Buszczak M, Freeman MR, Carlson JR et al. (1999) Ecdysone response genes govern egg chamber development during midoogenesis in Drosophila. Development 126: 4581–4589.

    CAS  PubMed  Google Scholar 

  • Chinzei Y, Chino H & Takahashi K (1983) Purification and properties of vitellogenin and vitellin from a tick, Ornithodoros moubata. J Comp Physiol B 152: 13–21.

    Article  CAS  Google Scholar 

  • Chinzei Y & Yano I (1985) Fat body is the site of vitellogenesis synthesis in the soft tick, Ornithodoros moubata. J Comp Physiol B 155: 671–678.

    Article  CAS  Google Scholar 

  • Chinzei Y (1986) Vitellogenin biosynthesis and processing in a soft tick. Host Regulated Development Mechanisms in Vector Arthropods, Proceedings of the Vero Beach Symposium (ed. by D Borovsky & A Spielman), pp 18–25. University of Florida-IFAS, Florida Medical Entomology Laboratory, Vero Beach, FL, USA.

    Google Scholar 

  • Chinzei Y & Taylor D (1990) Regulation of vitellogenesis induction by engorgement in the soft tick, Ornithodoros moubata. Adv Inverte Reprod 5: 565–570.

    Google Scholar 

  • Chinzei Y, Taylor D, Miura K & Ando K (1992) Vitellogenesis induction by a synganglion factor in adult female ticks Ornithodoros moubata (Acari: Argasidae). J Acarol Soc Jpn 1: 15–26.

    Article  Google Scholar 

  • Chinzei Y & Taylor D (1994) Hormonal regulation of vitellogenin synthesis in ticks. Advances in Disease Vector Research, volume 10 (ed. by KR Harris), pp 1–22. Springer, New York, NY, USA.

    Chapter  Google Scholar 

  • Christianson AM, King DL, Hatzivassiliou E et al.. (1992) DNA binding and heteromerization of the Drosophila transcription factor chorion factor 1/ultraspiracle. Proc Natl Acad Sci USA 89: 11503–11507.

    Article  Google Scholar 

  • Friesen KJ & Kaufman WR (2002) Quantification of vitellogenesis and its control by 20-hydroxyecdysone in the ixodid tick, Amblyomma hebraeum. J Insect Physiol 48: 773–782.

    Article  CAS  PubMed  Google Scholar 

  • Guo X, Harmon MA, Laudet V et al. (1997) Isolation of a functional ecdysteroid receptor homologue from the ixodid tick Amblyomma americanum (L.). Insect Biochem Mol Biol 127: 945–962.

    Article  Google Scholar 

  • Guo X, Xu Q, Harmon MA et al. (1998) Isolation of two functional retinoid X receptor subtypes from the Ixodid tick, Amblyomma americanum (L.). Mol Cell Endocrinol 139: 45–60.

    Article  CAS  PubMed  Google Scholar 

  • Hagedorn HH (1985) The role of ecdyteroids in reproduction. Comprehensive Insect Physiology Biochemistry and Pharmacology, volume 8 (ed. by GA Kerkut & LI Gilbert), pp 205–261. Pergamon Press, Oxford, UK.

    Google Scholar 

  • Hagedorn HH (1989) Physiological roles of hemolymph ecdysteroid in the adult insect. Ecdysone from Chemistry to Mode of Action (ed. by J Koolman), pp. 279–289. Thieme Medical Publishers, New York, NY, USA.

    Google Scholar 

  • Hall BL & Thummel CS (1998) The RXR homolog ultraspiracle is an essential component of the Drosophila ecdysone receptor. Development 125: 4709–4717.

    CAS  PubMed  Google Scholar 

  • Henrich VC & Brown NE (1995) Insect nuclear receptors: A developmental and comparative perspective. Insect Biochem Mol Biol 25: 881–897.

    Article  CAS  PubMed  Google Scholar 

  • Horigane M, Ogihara K, Nakajima Y et al. (2007a) Cloning and expression of the ecdysteroid receptor during ecdysis and reproduction in females of the soft tick, Ornithodoros moubata (Acari: Argasidae). Insect Mol Biol 16: 601–612.

    Article  CAS  PubMed  Google Scholar 

  • Horigane M, Ogihara K, Nakajima Y et al. (2007b) Identification and expression analysis of an actin gene from the soft tick, Ornithodoros moubata (Acari: Argasidae). Arch Insect Biochem Physiol 64: 186–199.

    Article  CAS  PubMed  Google Scholar 

  • Horigane M, Ogihara K, Nakajima Y & Taylor D (2008) Isolation and expression of the retinoid X receptor from last instar nymphs and adult females of the soft tick Ornithodoros moubata (Acari: Argasidae). Gen Comp Endocrinol 156: 298–311.

    Article  CAS  PubMed  Google Scholar 

  • James AM, Zhu XX & Oliver JH Jr (1997) Vitellogenin and ecdysteroid titers in Ixodes scapularis during vitellogenesis. J Parasitol 83: 559–563.

    Article  CAS  PubMed  Google Scholar 

  • Kapitskaya M, Wang S, Cress DE et al. (1996) The mosquito ultraspiracle homologue, a partner of ecdysteroid receptor heterodimer: cloning and characterization of isoforms expressed during vitellogenesis. Mol Cell Endocrinol 121: 119–132.

    Article  CAS  PubMed  Google Scholar 

  • Kokoza VA, Martin D, Mienaltowski MJ et al. (2001) Transcriptional regulation of the mosquito vitellogenin gene via a blood mealtriggered cascade. Gene 274: 47–65.

    Article  CAS  PubMed  Google Scholar 

  • Mangelsdorf DJ & Evans RM (1995) The RXR heterodimers and orphan receptors. Cell 83: 841–850.

    Article  CAS  PubMed  Google Scholar 

  • Mao H & Kaufman WR (1998) DNA binding properties of the ecdysteroid receptor in the salivary gland of the female ixodid tick, Amblyomma hebraeum. Insect Biochem Mol Biol 28: 947–957.

    Article  CAS  PubMed  Google Scholar 

  • Mao H & Kaufman WR (1999) Profile of the ecdysteroid hormone and its receptor in the salivary gland of the adult female tick, Amblyomma hebraeum. Insect Biochem Mol Biol 29: 33–42.

    Article  CAS  PubMed  Google Scholar 

  • Martin D, Wang SF & Raikhel AS (2001) The vitellogenin gene of the mosquito Aedes aegypti is a direct target of ecdysteroid receptor. Mol Cell Endocrinol 173: 75–86.

    Article  CAS  PubMed  Google Scholar 

  • McCall K (2004) Eggs over easy: cell death in the Drosophila ovary. Dev Biol 274: 3–14.

    Article  CAS  PubMed  Google Scholar 

  • Miura K, Wang SF & Raikhel AS (1999) Two distinct subpopulations of ecdysone receptor complex in the female mosquito during vitellogenesis. Mol Cell Endocrinol 156: 111–120.

    Article  CAS  PubMed  Google Scholar 

  • Oro AE, McKeown M & Evans RM (1990) Relationship between the product of the Drosophila ultraspiracle locus and the vertebrate retinoid X receptor. Nature 347: 298–301.

    Article  CAS  PubMed  Google Scholar 

  • Ogihara K, Horigane M, Nakajima Y et al. (2007) Ecdysteroid Hormone Titer and its Relationship to Vitellogenesis in the Soft Tick, Ornithodoros moubata (Acari: Argasidae). Gen Comp Endocrinol 150: 371–380.

    Article  CAS  PubMed  Google Scholar 

  • Palmer MJ, Warren JT, Jin X et al. (2002) Developmental profiles of ecdysteroids, ecdysteroid receptor mRNAs and DNA binding properties of ecdysteroid receptors in the Ixodid tick Amblyomma americanum (L.). Insect Biochem Mol Biol 32: 465–476.

    Article  CAS  PubMed  Google Scholar 

  • Raikhel AS, Kokoza VA, Zhu J et al. (2002) Molecular biology of mosquito vitellogenesis: from basic studies to genetic engineering of antipathogen immunity. Insect Biochem Mol Biol 32: 1275–1286.

    Article  CAS  PubMed  Google Scholar 

  • Rees HH (2004) Hormonal control of tick development and reproduction. Parasitology 129: 127–143.

    Article  Google Scholar 

  • Sauer JR, Essenberg RC & Bowman AS (2000) Salivary glands in ixodid ticks: control and mechanism of secretion. J Insect Physiol 46: 1069–1078.

    Article  CAS  PubMed  Google Scholar 

  • Sankhon N, Lockey T, Rosell RC et al. (1999) Effect of methoprene and 20-hydroxyecdysone on vitellogenin production in cultured fat bodies and backless explants from unfed female Dermacentor variabilis. J Insect Physiol 45: 755–761.

    Article  CAS  PubMed  Google Scholar 

  • Sonenshine DE (1994) Biology of Ticks, Volume 1. Oxford University Press, New York, NY, USA.

    Google Scholar 

  • Swevers L, Drevet JR, Lunke MD & Iatrou K (1995). The silk-moth homolog of the Drosophila ecdysone receptor (B1 isoform): cloning and analysis of expression during follicular cell differentiation. Insect Biochem Mol Biol 25: 857–866.

    Article  CAS  PubMed  Google Scholar 

  • Swevers L, Cherbas L, Cherbas P & Iatrou K (1996) Bombyx EcR (BmEcR) and Bombyx USP (BmCF1) combine to form a functional ecdysone receptor. Insect Biochem Mol Biol 26: 217–221.

    Article  CAS  PubMed  Google Scholar 

  • Taylor D, Chinzei Y & Ando K (1991) Vitellogenin synthesis, processing and hormonal regulation in the tick, Ornithodoros parkeri (Acari: Argasidae). Insect Biochem 21: 723–733.

    Article  CAS  Google Scholar 

  • Taylor D, Moribayashi A, Agui N et al. (1997) Hormonal regulation of vitellogenesis in the soft tick, Ornithodoros moubata. Advances in Comparative Endocrinology (ed. by S Kawashima & S Kikuyama), pp 213–220. Monduzzi Editore, Bologna, Italy.

    Google Scholar 

  • Taylor D & Chinzei Y (2002) Vitellogenesis in the tick. Recent Progress in Vitellogenesis - Reproductive Biology of Invertebrates, volume 12 (ed. by AS Raikhel & TW Sappington), pp 175–199. Science Publishers, Enfield, NH, USA.

    Google Scholar 

  • Thomas HE, Stunnenberg HG & Stewart AF (1993) Heterodimerization of the Drosophila ecdysone receptor with retinoid X receptor and ultraspiracle. Nature 362: 471–475.

    Article  CAS  PubMed  Google Scholar 

  • Thompson DM, Khalil SM, Jeffers LA et al. (2005) In vivo role of 20- hydroxyecdysone in the regulation of the vitellogenin mRNA and egg development in the American dog tick, Dermacentor variabilis (Say). J Insect Physiol 51: 1105–1116.

    Article  CAS  PubMed  Google Scholar 

  • Wang SF, Li C, Zhu J et al. (2000) Differential expression and regulation by 20-hydroxyecdysone of mosquito ultraspiracle isoforms. Dev Biol 218: 99–113.

    Article  CAS  PubMed  Google Scholar 

  • Wang SF, Li C, Sun G et al. (2002) Differential expression and regulation by 20-hydroxyecdysone of mosquito ecdysteroid receptor isoforms A and B. Mol Cell Endocrinol 196: 29–42.

    Article  CAS  PubMed  Google Scholar 

  • Yao TP, Segraves WA, Oro AE et al. (1992) Drosophila ultraspiracle modulates ecdysone receptor function via heterodimer formation. Cell 71: 63–72.

    Article  CAS  PubMed  Google Scholar 

  • Yao TP, Forman BM, Jiang Z et al. (1993) Functional ecdysone receptor is the product of EcR and Ultraspiracle genes. Nature 366: 476–479.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8572, Japan

    Mari Horigane & DeMar Taylor

Authors
  1. Mari Horigane
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  2. DeMar Taylor
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Correspondence to DeMar Taylor .

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Editors and Affiliations

  1. Section of Population Biology, Institute for Biodiversity and Ecosystem, Amsterdam, 1090 GE, Netherlands

    Maurice W. Sabelis

  2. Section Population Biology, Institute for Biodiversity and Ecosystem, Amsterdam, 1090 GE, Netherlands

    Jan Bruin

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Horigane, M., Taylor, D. (2010). Analysis of tissues for EcR and RXR nuclear receptor gene expression during vitellogenesis in the soft tick Ornithodoros moubata . In: Sabelis, M., Bruin, J. (eds) Trends in Acarology. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9837-5_36

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