Abstract
We found that the increased expression of fructose transporter, GLUT5 improved cell proliferation and antibody production in human hybridoma and myeloma cell lines cultured in the fructose-based medium. The IgG and GLUT5 expression vectors cotransfected myeloma clones showed a tendency to increase IgG production in the fructose-based medium more than in the glucose-based medium. The both IgG and GLUT5 expression vector transfected myeloma cells increased its tendency, suggesting that IgG expression might be activated with GLUT5 expression in the fructose-based medium when IgG gene was located in the vicinity of GLUT5 gene.
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Tsukamoto, Y., Nakamura, S., Inoue, A., Nishino, N., Inoue, Y., Kawahara, H. (2010). Development of Protein High Expression System by Using Fructose Metabolism. In: Kamihira, M., Katakura, Y., Ito, A. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 16. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3892-0_19
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DOI: https://doi.org/10.1007/978-90-481-3892-0_19
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