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Establishment of Transplantable Biliary Cancer Lines

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Book cover Hepatobiliary and Pancreatic Carcinogenesis in the Hamster

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A transplantable cancer line from the gallbladder was established in Syrian golden hamsters. The primary tumor of the gallbladder was induced by a subcutaneous injection of N-nitrosobis(2-oxopropyl)amine (BOP) in hamsters after cholecystoduodenostomy with dissection of the common duct. The tumor was inoculated into the cavity of the gallbladder and subcutaneous tissue and was transplantable, with an uptake rate of 100%. Histologically, the primary induced gallbladder tumor was well-differentiated papillary adenocarcinoma and the principal structure of all allograft tumors was of poorly differentiated ductal adenocarcinoma. These tumors expressed blood group-related antigens, including A, H, and P-glycoproteins. To our knowledge, this is the first report of carcinoma of the gallbladder transplantable in a laboratory animal.

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Author information

Authors and Affiliations

  1. Department of Surgery, Aino Memorial Hospital, 838-1 Aino, Unzen, Nagasaki, 854-0301, Japan

    Tomohiro Fukahori

  2. Department of Surgery, Nagasaki Municipal Hospital, 6-39 Shinchi, Nagasaki, 850-8555, Japan

    Keiji Inoue

  3. Department of Surgery, Nagasaki Yurino Hospital, 1155-2 Motomura, Togitsu, Nishisonogi, Nagasaki, 851-2103, Japan

    Tsutomu Tomioka

  4. Department of Surgery, Division of Gastroenterological Surgery, Kawasaki Medical School, 577 Matsushima, Kurashiki, Okayama, 701-0192, Japan

    Tsukasa Tsunoda

Authors
  1. Tomohiro Fukahori
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  2. Keiji Inoue
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  3. Tsutomu Tomioka
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  4. Yoshitsugu Tajima
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  5. Tsukasa Tsunoda
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  6. Takashi Kanematsu
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Editors and Affiliations

  1. Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan

    Yoshitsugu Tajima M.D., Ph.D. , Tamotsu Kuroki M.D., Ph.D.  & Takashi Kanematsu M.D., Ph.D. ,  & 

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Fukahori, T., Inoue, K., Tomioka, T., Tajima, Y., Tsunoda, T., Kanematsu, T. (2009). Establishment of Transplantable Biliary Cancer Lines. In: Tajima, Y., Kuroki, T., Kanematsu, T. (eds) Hepatobiliary and Pancreatic Carcinogenesis in the Hamster. Springer, Tokyo. https://doi.org/10.1007/978-4-431-87773-8_13

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