Abstract
Members of the family Poxviridae form a large group of viruses that can infect humans as well as animals including the major domestic animal species (cattle, sheep, goat, swine, dog, cat and chicken). Poxviruses can be highly pathogenic for humans (i.e., Variola virus), of zoonotic importance (e.g., Monkeypox virus) or highly contagious among animal populations (e.g., Sheeppox virus). Therefore, laboratory confirmation of the specific poxvirus involved is, indeed, essential. This is especially true for the most notorious member, Variola virus, the smallpox virus, which might reemerge as a weapon, and also for those “exotic” poxviruses which are absent in many countries but still enzootic in other parts of the world. Today, poxvirus diagnostics covers the entire spectrum of either traditional (such as inoculation of embryonated eggs) or more advanced laboratory tests (such as genome sequencing or microarray assays). This chapter presents methods of sample collection and handling, and reviews techniques used in the diagnosis of poxvirus infections by briefly describing the principle and procedure of the method, and critically weighting the pros and cons as well as providing some examples of application for each method.
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References
Damon IK, Esposito JJ (2003) Poxviruses that infect humans. In: PR Murray, EJ Baron, JH Jorgensen, MA Pfaller, MH Yolker (eds): Manual of clinical microbiology, 8th edn. ASM Press, Washington DC, 1583–1591
Henderson DA, Inglesby TV, Bartlett JG, Ascher MS, Eitzen E, Jahrling PB, Hauer J, Layton M, McDade J, Osterholm TM et al (1999) Smallpox as a biological weapon: medical and public health management. Working Group on Civilian Biodefense. JAMA 281: 2127–2137
Hazelton PR, Gelderblom HR (2003) Electron microscopy for rapid diagnosis of infectious agents in emergent situations. Emerg Infect Dis 9: 294–303
Renner-Müller IC, Meyer H, Munz E (1995) Characterization of camelpoxvirus isolates from Africa and Asia. Vet Microbiol 45: 371–381
Downie AW (1939) A study of the lesions produced experimentally by cowpox virus. J Pathol 48: 361–378
Meyer H, Ropp SL, Esposito JJ (1997) Gene for A-type inclusion body protein is useful for a polymerase chain reaction assay to differentiate orthopoxviruses. J Virol Methods 64: 217–221
Tripathy DN, Hanson LE (1976) A smear technique for staining elementary bodies of fowlpox. Avian Dis 20: 609–610
Lazarus AS, Eddie A, Meyer KF (1937) Propagation of variola virus in the developing egg. Proc Soc Exp Biol Med 36: 7–8
Samour JH, Kaaden OR, Wernery U, Bailey TA (1996) An epornitic of avian pox in houbara bustards (Chlamydotis undulata macqueenii). Zentralbl Veterinaermed B 43: 287–292
Niikura M, Narita T, Mikami T (1991) Establishment and characterization of a thymidine kinase deficient avian fibroblast cell line derived from a Japanese quail cell line, QT35. J Vet Med Sci 53: 439–446
Czerny C-P, Meyer H, Mahnel H (1989) Establishment of an ELISA for the detection of orthopox viruses based on neutralizing monoclonal and polyclonal antibodies. Zentralbl Veterinarmed B 36: 537–546
Carn VM (1995) An antigen trapping ELISA for the detection of capripoxvirus in tissue culture supernatant and biopsy samples. J Virol Methods 51: 95–102
Bults HG, Brandon MR (1982) An immunological method to determine antigenic variation in three strains of myxoma virus. J Virol Methods 5: 21–26
Hsu SM, Raine L (1981) Protein A, avidin, and biotin in immunohistochemistry. J Histochem Cytochem 29: 1349–1353
Mackett M, Archard LC (1979) Conservation and variation in orthopoxvirus genome structure. J Gen Virol 45: 683–701
Esposito JJ, Knight JC (1985) Orthopoxvirus DNA: a comparison of restriction profiles and maps. Virology 143: 230–251
Naidoo J, Baxby D, Bennett M, Gaskell RM, Gaskell CJ (1992) Characterization of orthopoxviruses isolated from feline infections in Britain. Arch Virol 125: 261–272
Meyer H, Schay C, Mahnel H, Pfeffer M (1999) Characterization of orthopoxviruses isolated from man and animals in Germany. Arch Virol 144: 491–501
Neubauer H, Reischl U, Ropp S, Esposito JJ, Wolf H, Meyer H (1998) Specific detection of monkeypox virus by polymerase chain reaction. J Virol Methods 74: 201–207
Ropp SL, Jin Q, Knight JC, Massung RF, Esposito JJ (1995) PCR strategy for identification and differentiation of small pox and other orthopoxviruses. J Clin Microbiol 33: 2069–2076
Loparev VN, Massung RF, Esposito JJ, Meyer H (2001) Detection and differentiation of old world orthopoxviruses: restriction fragment length polymorphism of the crmB gene region. J Clin Microbiol 39: 94–100
Heine HG, Stevens MP, Foord AJ, Boyle DB (1999) A capripoxvirus detection PCR and antibody ELISA based on the major antigen P32, the homolog of the vaccinia virus H3L gene. J Immunol Methods 30: 187–196
Luschow D, Hoffmann T, Hafez HM (2004) Differentiation of avian poxvirus strains on the basis of nucleotide sequences of 4b gene fragment. Avian Dis 48: 453–462
Nunez A., Funes JM, Agromayor M, Moratilla M, Varas AJ, Lopez-Estebaranz JL, Esteban M, Martin-Gallardo A (1996) Detection and typing of molluscum contagiosum virus in skin lesions by using a simple lysis method and polymerase chain reaction. J Med Virol 50: 342–349
Thompson CH (1997) Identification and typing of molluscum contagiosum virus in clinical specimens by polymerase chain reaction. J Med Virol 53: 205–211
Torfason EG, Gunadottir S (2002) Polymerase chain reaction for laboratory diagnosis of orf virus infections. J Clin Virol 24: 79–84
Espy MJ, Cockerill IF, Meyer RF, Bowen MD, Poland GA, Hadfield TL, Smith TF (2002) Detection of smallpox virus DNA by LightCycler PCR. J Clin Microbiol 40: 1985–1988
Ibrahim MS, Esposito JJ, Jahrling PB, Lofts RS (1997) The potential of 5’ nuclease PCR for detecting a single-base polymorphism in orthopoxvirus. Mol Cell Probes 11: 143–147
Ibrahim MS, Kulesh DA, Saleh SS, Damon IK, Esposito JJ, Schmaljohn AL, Jahrling PB (2003) Real-time PCR assay to detect smallpox virus. J Clin Microbiol 41: 3835–3839
Panning M, Asper M, Kramme S, Schmitz H, Drosten C (2004) Rapid detection and differentiation of human pathogenic orthopox viruses by a fluorescence resonance energy transfer real-time PCR assay. Clin Chem 50: 702–708
Nitsche A, Ellerbrok H, Pauli G (2004) Detection of orthopoxvirus DNA by real-time PCR and identification of variola virus DNA by melting analysis. J Clin Microbiol 42: 1207–1213
Kulesh DA, Baker RO, Loveless BM, Norwood D, Zwiers SH, Mucker E, Hartmann C, Herrera R, Miller D, Christensen D et al (2004) Smallpox and pan-orthopox virus detection by real-time 3’-minor groove binder TaqMan assays on the Roche LightCycler and the Cepheid smart Cycler platforms. J Clin Microbiol 42: 601–609
Olson VA, Laue T, Laker MT, Babkin IV, Drosten C, Shchelkunov SN, Niedrig M, Damon IK, Meyer H (2004) Real-time PCR system for detection of orthopoxviruses and simultaneous identification of smallpox virus. J Clin Microbiol 42: 1940–1946
Zimmermann P, Thordsen I, Frangoulidis D, Meyer H (2005) Real-time PCR assay for the detection of tanapox virus and yaba-like disease virus. J Virol Methods 130: 149–153
Lapa S, Mikheev M, Shchelkunov S, Mikhailovich V, Sobolev A, Blinov V, Babkin L, Guskov A, Sokunova E, Zasedatelev A et al (2002) Species-level identification of orthopoxviruses with an oligonucleotide microchip. J Clin Microbiol 40: 753–757
Laassri M, Chizhikov V, Mikheev M, Shchelkunov S, Chumakov K (2003) Detection and discrimination of orthopoxviruses using microarrays of immobilized oligonucleotides. J Virol Methods 112: 67–78
Gubser C, Hue S, Kellam P, Smith GL (2004) Poxvirus genomes: a phylogenetic analysis. J Gen Virol 85: 105–117
Likos AM, Sammons SA, Olson VA, Frace AM, Li Y, Olsen-Rasmussen M, Davidson W, Galloway R, Khristova ML, Reynolds MG et al (2005) A tale of two clades: monkeypox viruses. J Gen Virol 86: 2661–2672
Kaerber G (1931) DeBeitrag zur kollektiven Behandlung pharmakologischer Reihenversuche. Arch Exp Pathol Pharmakol 162: 480
Czerny C-P, Wagner K, Gessler K, Mayr A, Kaaden O-R (1996) A monoclonal blocking ELISA for detection of orthopox virus antibodies in feline sera. Vet Microbiol 52: 185–200
Karem KL, Reynolds M, Braden Z, Lou G, Bernard N, Patton J, Damon IK (2005) Characterization of acute-phase humoral immunity to monkeypox: use of immunoglobulin M enzyme-linked immunosorbent assay for detection of monkeypox infection during the 2003 North American outbreak. Clin Diag Lab Immunol 12: 867–872
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Pfeffer, M., Meyer, H. (2007). Poxvirus diagnostics. In: Mercer, A.A., Schmidt, A., Weber, O. (eds) Poxviruses. Birkhäuser Advances in Infectious Diseases. Birkhäuser Basel. https://doi.org/10.1007/978-3-7643-7557-7_17
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DOI: https://doi.org/10.1007/978-3-7643-7557-7_17
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