Abstract
Background: The individual difference of cerebral vasospasm (CVS) degree after subarachnoid hemorrhage (SAH) is common in clinic observation. Numerous studies have found that early CVS after SAH is associated with derangements in catecholamine(CA) metabolism. Catechol-O-methyltransferase (COMT) is a key rate-limiting enzyme in the degradation of CA. In this study, we investigate the correlation between COMT gene polymorphism of patients and early CVS after SAH.
Methods: One hundred and sixty-seven patients with spontaneous SAH in early stage were selected in this study. COMT genotyping was performed by means of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The degree of CVS was identified by TCD. Hunt–Hess classification was used to evaluate the severity of the patients’ condition. The bleeding amount was evaluated by means of Fisher classification of head CT. χ2 test (SPSS13.0 software) and logistic regression were adopted to analyze the correlation of COMT gene polymorphism and other clinical data of patients with early CVS after SAH.
Results: The distribution of each allele matched Hardy–Weinberg law and research samples were heredity equilibrium population. Early CVS incidence of patients with COMT-A allele was much higher than those with COMT-G allele (P<0.01). Early CVS incidence of patients with COMT A/A genotype was obviously higher than those with COMT G/G genotype (P<0.05). Univariate logistic regression demonstrated that COMT-A allele, A/A genotype and Grade 3–5 of Hunt–Hess classification were all associated with early CVS. After adjustment of general information, further multivariate logistic regression demonstrated that COMT-A allele, A/A genotype were risk factors of early CVS after SAH.
Conclusion: COMT-A allele, A/A genotype were risk factors of early CVS after SAH.
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Dilraj A, Botha JH, Rambiritch V, Miller R, van Dellen JR. Levels of catecholamine in plasma and cerebrospinal fluid in aneurysmal subarachnoid hemorrhage. Neurosurgery 1992;31(1):42–50.
Naredi S, Lambert G, Edén E, Zäll S, Runnerstam M, Rydenhag B, et al. Increased sympathetic nervous activity in patients with nontraumatic subarachnoid hemorrhage. Stroke 2000;31:901–6.
Condette-Auliac S, Bracard S, Anxionnat R, Schmitt E, Lacour JC, Braun M, et al. Vasospasm after SAH: interest in diffusion-weighted MR imaging. Stroke 2001;32:1818–24.
Shen JK. The pathogenesis of cerebral vasospasm and its prevention and treatment. Int J Cerebrovasc Dis. 2006;14(7):481–93.
Ostrowski RP, Colohan AR, Zhang JH. Molecular mechanisms of early brain injury after subarachnoid hemorrhage. Neurolog Res. 2006;28:399–414.
Khurana VG, Sohni YR, Mangrum WI, McClelland RL, O(Kane DJ, Meyer FB, et al. Section on cerebrovascular surgery: Galbraith award: endothelial nitric oxide synthase (eNOS) and heme oxygenase-1 (HO-1) gene polymorphisms predict susceptibility to aneurysmal subarachnoid hemorrhage (SAH) and post-SAH cerebral vasospasm. Clin Neurosurg. 2004;51:343–50.
Ko NU, Rajendran P, Kim H, Rutkowski M, Pawlikowska L, Kwok PY, et al. Endothelial nitric oxide synthase polymorphism (-786T- > C) and increased risk of angiographic vasospasm after aneurysmal subarachnoid hemorrhage. Stroke 2008;39(4):1103–8.
Kunugi H, Nanko S, Ueki A, Otsuka E, Hattori M, Hoda F, et al. High and low activity alleles of catechol-O-methyltransferase gene: ethnic difference and possible association with Parkinson′s disease. Neurosci Lett. 1997;221:202–4.
Goldberg TE, Egan MF, Gscheidle T, Coppola R, Weickert T, Kolachana BS. Executive subprocesses in working memory: relationship to catechol-O-methyltransferase Val158Met genotype and schizophrenia. Arch Gen Psychiatry. 2003;60:889–96.
Eisenberg J, Mei-Tal G, Steinberg A, Tartakovsky E, Zohar A, Gritsenko I, et al. Haplotype relative risk study of catechol-o-methyltransferase (COMT) and attention deficit hyperactivity disorder (ADHD): association of the high-enzyme activity Valallele with ADHD impulsive-hyperactive phenotype. Am J Med Gene. 1999;88:497–502.
Kochanowicz J, Krejza J, Mariak Z, Ustymowicz A, Lewko J. Diagnosis of middle cerebral artery spasm by determination of flow velocity and the Lindegaard index with transcranial color Doppler sonography. Neurol Neurochir Pol. 2005;39:11–6.
Lindegaard KF. The role of transcranial Doppler in the management of patients with subarachnoid haemorrhage: a review. Acta Neurochir (Wien). 1999;72:59–71.
Aaslid R, Huber R, Nomes H. Evaluation of cerebrovascular spasm with transcranial Doppler ultrasound. J Neurosurg. 1984;60:37–42.
Harders AG, Gilsbach JM. Time course of blood velocity changes related to vasospasm in the circle of Willis measured by transcranial Doppler ultrasound. J Neurosurg. 1987;66:718–22.
Aäslid R et al. Transcranial doppler sonography. New York: Springer; 1986.
Grosset DG, Straiton J, McDonald I, Bullock R. Angiographic and Doppler diagnosis of cerebral artery vasospasm following subarachnoid Hemorrhage. Br J Neurosurg. 1993;7(3):291–8.
Aaslid R. Transcranial Doppler assessment of cerebral vasospasm. Eur J Ultrasound. 2002;16:3–10.
Männistö PT, Kaakkola S. Catechol-O-methyltransferase (COMT): biochemistry molecular biology, pharmacology, and clinical efficacy of the new selective COMT inhibitors. Pharmacol Rev. 1999;51(4):594–622.
Chen X, Wang X, O(Neill AF, Walsh D, Kendler KS. Variants in the catechol-o-methyltransferase (COMT) gene are associated with schizophrenia in Irish high – density families. Mol Psychiatry. 2004;9:(10):962–7.
Spielman RS, Weinshilboum RM. Genetics of red cell COMT activity: analysis of thermal stability and family data. Am J Med Genet. 1981;10:279–90.
Lotta T, Vidgren J, Tilgmann C, Ulmanen I, Melén K, Julkunen I, et al. Kinetics of human soluble and membrane-bound catechol-O-methyltransferase: a revised mechanism and description of the thermolabile variant of the enzyme. Biochemisty 1995;34:4202–10.
Palmatier MA, Kang AM, Kidd KK. Global variation in the frequencies of functional different catechol-O-methyltransferase alleles. Biol Psychiatry. 1999;46:557–67.
Janjua N, Mayer SA. Cerebral vasospasm after subaracnoid hemorrhage. Curr Opin Crit Care. 2003;9(2):113–9.
Hirashima Y, Kurimoto M, Hori E, Origasa H, Endo S. Lower incidence of symptomatic vasospasm after subarachnoid hemorrhage owing to ruptured vertebrobasilar aneurysms. Neurosurgery 2005;57(6):1110–6.
Dietrich HH, Dacey RG Jr. Molecular keys to the problems of cerebral vasospasm. Neurosurgery 2000;46:517–30.
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Financial support from the Nature Science Funds of Chongqing Science and Technology Committee (CSTC, 2008BB5219) is gratefully acknowledged.
Conflict of interest statement We declare that we have no conflict of interest.
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He, Z., Sun, X., Guo, Z., Zhang, J.H. (2011). The Correlation Between COMT Gene Polymorphism and Early Cerebral Vasospasm After Subarachnoid Hemorrhage. In: Feng, H., Mao, Y., Zhang, J.H. (eds) Early Brain Injury or Cerebral Vasospasm. Acta Neurochirurgica Supplements, vol 110/1. Springer, Vienna. https://doi.org/10.1007/978-3-7091-0353-1_41
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