Zusammenfassung
D-β-Hydroxybutyric dehy drogenase of respiratory particles from pig heart was first described in 1937 by Green, Dewan and Leloir 1 . With an assay based on oxygen uptake through the cytochrome system, they demonstrated that the enzyme was stereospecific for the D-isomer and that β-hydroxypropionic acid, αhydroxybutyric acid, γ-hydroxybutyric acid, crotonic acid, butyric acid, and acetic acid were not oxidized under conditions in which β-hydroxybutyrate was oxidized vigorously. The enzyme was shown to be distinct from malic and lactic dehydrogenases. The particulate enzyme also dehydrogenates higher d-β-hydroxy aliphatic acids up to nine carbon atoms long2, but does not act on l-β-hydroxyacids or their coenzyme A thioesters3. d-β-hydroxybutyric dehydrogenase is specific for DPN and will not reduce TPN1,3 or desamino-DPN4.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Literatur
Green, D. E., J. G. Dewan and L. F. Leloir: Biochem. J. 31, 934 (1937).
Lang, K.: H. 277, 114 (1942).
Lehninger, A. L., H. C. Sudduth and J. B. Wise: J. biol. Ch. 235, 2450 (1960).
Pullman, M. E., S. P. Colowick and N. O. Kaplan: J. biol. Ch. 194, 593 (1952).
Nachlas, M. M., D. G. Walker and A. M. Seligman: J. biophys. biochem. Cytol. 4, 29 (1958).
Brodie, A. F., and C. T. Gray: J. biol. Ch. 219, 853 (1956).
Emmelot, P., and C. J. Bos: Enzymologia 18, 179 (1957).
Jensen, C. O., W. Sacks and F. A. Baldauski: Science, N. Y. 113, 65 (1951).
Sacktor, B.: J. biophys. biochem. Cytol. 1, 29 (1955).
Lehninger, A. L., H. C. Sudduth and J. B. Wise: J. biol. Ch. 235, 2450 (1960).
Schneider, W. C.: J. biol. Ch. 176, 259 (1948).
Hoff-Jorgenson, E.: Skand. Arch. Physiol. 80, 176 (1938).
Burton, K., and T. H. Wilson: Biochem. J. 54, 86 (1953).
Lardy, H. A., D. Johnson and W. C. McMurray: Arch. Biochem. 78, 587 (1958).
Lehninger, A. L.: J. biol. Ch. 190, 345 (1951).
Wise, J. B., and A. L. Lehninger: J. biol. Ch. 237, 1363 (1962).
Hokecker, B. L., and A. Kornberg: J. biol. Ch. 175, 385 (1948).
Nachlas, M. M., D. G. Walker and A. M. Seligman: J. biophys. biochem. Cytol. 4, 29 (1958).
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 1964 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Wise, J.B., Lehninger, A.L. (1964). D-β-Hydroxybutyric dehydrogenase. In: Abraham, R., et al. Enzyme. Handbuch der Physiologisch- und Pathologisch-Chemischen Analyse, vol 6 / a. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-11689-0_11
Download citation
DOI: https://doi.org/10.1007/978-3-662-11689-0_11
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-662-11690-6
Online ISBN: 978-3-662-11689-0
eBook Packages: Springer Book Archive