Abstract
The in vitro semisolid agar culture system recently developed by Hamburger and Salmon favors the cloning of human tumor cells (Salmon et al. 1978). Experience in several laboratories indicates that some tumor types, such as malignant melanomas, ovarian adenocarcinomas, and neuroblastomas, seem to grow better than, for instance, sarcomas and colorectal and non-small-cell bronchogenic carcinomas (Von Hoff 1981). Because successful in vitro cloning is critically dependent on fresh biopsies, experiments are performed only once. Due to these practical constraints, information concerning the reproducibility and interlaboratory performance of the assay is not available. Such comparisons would be possible if ongoing studies in other centers could demonstrate that cryopreserved tumor specimens retain the biological and pharmacologic properties of the fresh samples. Thus far, however, this information is not available.
V.H. and U.F. were supported by the EMDO Foundation. E.E.H. was supported by grant SNF 3.873.0.79 and the Regional Cancer Leagues St. Gallen-Appenzell and Thurgau
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© 1984 Springer-Verlag Berlin · Heidelberg
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Hofmann, V., Holdener, E.E., Müller, M., Früh, U. (1984). Interlaboratory Comparison of In Vitro Cloning of Fresh Human Tumor Cells from Malignant Effusions. In: Hofmann, V., Berens, M.E., Martz, G. (eds) Predictive Drug Testing on Human Tumor Cells. Recent Results in Cancer Research, vol 94. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-82295-7_20
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DOI: https://doi.org/10.1007/978-3-642-82295-7_20
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