Abstract
Since the first reports on altered urinary excretion patterns of nucleic acid catabolites by humans suffering from various diseases (Weissman et al. 1962, 1963; Mirvish et al. 1971; Pinkard et al. 1972), more and more evidence has accumulated that cancer patients excrete increased amounts of these compounds (Cooper et al. 1977; Schöch and Heller-Schöch 1977; Gehrke et al. 1978; Gehrke et al. 1979). The specificity in the increased excretion rates of the RNA catabolites is demonstrated by the fact that patients suffering from non-cancer diseases, such as acute infections and chronic pulmonary diseases, do not have altered excretion rates of modified RNA constituents (Waalkes et al. 1975; Schöch et al. 1979).
We are very grateful to Privat-Dozent Dr. Arne Luz, Institut fur Biologie der GSF, Abteilung fur Pathologie, Neuherberg, for carrying out the histological examinations and interpreting the findings. We also wish to thank Petra Micheels for excellent technical assistance
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Abbreviations
- MCA:
-
3-methylcholanthrene
- HPLC:
-
high-pressure liquid chromatography
- RSD:
-
relative standard deviation
- SD:
-
standard deviation
- m1A:
-
1-methyladenosine
- ac4C:
-
N 4-acetylcytidine
- m5C:
-
5-methylcytidine
- m1G:
-
1-methylguanosine
- m2G:
-
N 2-methylguanosine:
- m 22 G:
-
N 2, N 2-di-methylguanosine
- m1l:
-
1-methylinosine
- ψ:
-
5-(ß-D-ribofuranosyl)-uracil (pseudouridine)
- m1Gua:
-
1-methylguanine
- m7Gua:
-
7-methylguanine
- m2Gua:
-
N 2-melhylguanine
- m 22 U:
-
N 2,N 2-dimethylguanine
References
Borek E, Baliga BS, Gehrke CW, Kuo KC, Belman S, Troll W, Waalkes TP (1977) High turnover of tRNA in tumor tissue. Cancer Res 37:3362–3366
Burt ME, Lowry SF, Gorschboth C, Brennan MF (1981) Metabolic alterations in a noncachectic animal tumor system. Cancer 47:2138–2146
Chu BCF, Lawley PD (1975) Increased urinary excretion of nucleic acid and nicotinamide derivatives by rats after treatment with alkylating agents. Chem Biol Interactions 10:333–338
Cooper IA, Wray GR, Murphy TL (1977) Urinary exeretory patterns of tRNA degradation products: A marker of Hodgkin’s cell metabolism? Eur J Cancer 13:1309–1312
Gauss DH, Sprinzl M (1981) Compilation of tRNA sequences. Nucleic Acids Res 9:rl–r23
Gehrke, CW, Kuo KC (1981) Major and modified nucleosides, RNA and DNA. In: Marton LJ, Kabra PM (eds) Liquid chromatography in clinical analysis. Humana Press, Clifton pp. 409–444
Gehrke, CW, Kuo KC, Davis GE, Suits RD, Waalkes TP, Borek E (1978) Quantiative high-performance liquid chromatography of nucleosides in biological materials. J Chro-matogr 150:455–476
Gehrke CW, Kuo KC, Waalkes TP, Borek E (1979) Patterns of urinary excretion of modified nucleosides. Cancer Res 39:1150–1153
Hanski C, Stehlik G (1980) Increased concentration of 7-methylguanine and 1-methylhypoxan-thine in urine of rats bearing Yoshida tumour. Cancer Lett 9:339–343
Lakings, DB, Waalkes TP, Borek E, Gehrke CW, Mrochek JE, Longmore J, Adamson RH (1977) Composition, associated tissue methyltransferase activity, and catabolic end products of transfer RNA from carcinogen-induced Hepatoma and normal monkey livers. Cancer Res 37:285–292
Marini M, Muldoon WP, Mushinsky JF (1979) Biochemical changes induced by tumors at distant sites: Altered transfer RNA profiles in liver of mice bearing plasmacytomas. Cancer Lett 9:177–181
McFarlane ES, Shaw GJ (1968) Observed increase in methylated purines excreted by hamsters bearing adenovirus-12 induced tumors. Can J Microbiol 14:185
Mirvish SS, Medalie J, Linsell CA, Yousuf E, Reyad S (1971) 7-Methylguanine and other minor urinary purines: values for normal subjects from Israel, Gaza, and Kenya, and for patients with Cancer of various organs or cirrhosis of the liver. Cancer 27: 736–743
Pinkard PJ, Cooper IA, Motteram R, Turner CN (1972) Purine and pyrimidine excretion in Hodgkin’s disease. J Natl Cancer Inst 49:27–37
Sachs L (1979) Statistische Methoden. Springer, Berlin Heidelberg New York
Schöch G, Heller-Schöch G (1977) Molekularbiologie und klinische Bedeutung des Stoff-wechsels normaler und modifizierter Nucleobasen. Helv Paediatr Acta [Suppl] 38:7–171
Schöch G, Thomale J, Lorenz H, Suberg H, Karsten U (1980) A new method for the simultaneous analysis of unmodified and modified urinary nucleosides and nucleobases by high Performance liquid chromatography. Clin Chim Acta 108:247–257
Schöch G, Winkler K, Heller-Schöch G, Baisch H (1979) Die Analyse von normalen und methylierten Nucleobasen im Urin als neues Kriterium für Diagnose und Verlauf von Malignomen. Klin Pädiatr 191:197–204
Shimizu M, Fujimura S (1978) Studies on the abnormal excretion of pyrimidine nucleosides in the urine of Yoshida ascites sarcoma-bearing rats. Increased excretion of deoxycytidine, pseudouridine and cytidine. Biochim Biophys Acta 517:277–286
Thomale J, Nass G (1982) Elevated urinary excretion of RNA eatabolites as an early signal of tumor development in mice. Cancer Lett 15:149–159
Upton AC, Wolff FF, Furth J, Kimball AW (1958) A comparison of the induction of myeloid and lymphoid leukemias in X-radiated RF mice. Cancer Res 18:842–848
Waalkes TP, Gehrke CW, Zumwalt RW, Chang SY, Lakings DB, Tormey DC, Ahmann DL, Moertel CG (1975) The urinary excretion of nucleosides of ribonucleic acid by patients with advanced Cancer. Cancer 36:390–398
Weber G (1977) Enzymology of Cancer cells (part II). N Engl J Med 296: 541–551
Weissman S, Eisen AZ, Karon M (1962) Pseudouridine metabolism. IL Urinary excretion in gout, psoriasis, leukemia, and heterozygous oroticaciduria. J Lab Clin Med 59:852–858
Weissman S, Lewis M, Karon M (1963) Pseudouridine metabolism. IV. Excretion of pseudouridine and other nitrogenous metabolites in chronic leukemia. Blood 22: 657–663
Weyland P, Gross HJ, Dannenberg H (1972) Ausscheidung von 7-Methylguanin im Harn von Ratten nach einmaliger Applikation verschiedener Carcinogene. Z Krebsforsch 77:141–149
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© 1983 Springer-Verlag Berlin • Heidelberg
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Thomale, J., Nass, G. (1983). Increasing Urinary Levels of Modified Nucleosides and Bases During Tumor Development in Mice. In: Nass, G. (eds) Modified Nucleosides and Cancer. Recent Results in Cancer Research/Fortschritte der Krebsforschung/Progrès dans les recherches sur Ie cancer, vol 84. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-81947-6_28
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DOI: https://doi.org/10.1007/978-3-642-81947-6_28
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