Abstract
Depending on the source of mammalian cells and their growth state, 11 isoaccepting tRNAs for lysine can be demonstrated by RPC-5 chromatography or in combination with polyacrylamide gel electrophoresis. A composite profile illustrating these isoacceptors is shown in Fig. 1. The isoacceptors 1, 2, 5a, and 5 are observed in tRNA preparations from most mammalian tissues with insignificant variations from tissue to tissue. In contrast, tRNA Lys4 is present in greater amounts in growing cells than in resting cells (Ortwerth and Liu 1973; Juarez et al. 1975). Variations in amounts of tRNA Lys4 are correlated with the ability of cells to divide, and the isoacceptor was proposed as a requirement for cell division (Ortwerth 1971). It is not detectable in permanently differentiated tissues. The level of tRNA Lys4 is directly related to the doubling time of cells and drops sharply just prior to confluency (Conlon-Hollingshead and Ortwerth 1980; Katze 1975; Ortwerth 1971). Isoacceptors 2b, 3, 4a, 4b, and 5b are not observed routinely, either because of their presence in small amounts and cochromatography with other isoacceptors or because they occur only in certain tissues. tRNA Lys4a is not shown in the profile, but it elutes with tRNA Lys4 . tRNA Lys6 occurs in some cell lines transformed by viruses; polyoma-virus-transformed 3T3 and Balb/3T3 (Jacobson et al. 1974), murine sarcoma virus transformed Balb/3T3 (Hedgcoth et al. 1980a, b), and Rous-sarcoma-virus-transformed NRK (C. Hedgcoth, K. Scheets, and S. Allen, unpublished results). This isoacceptor has also been observed in some cell lines not known to be transformed by viruses (Chu-Der and Ortwerth 1980).
This work was supported by grants CA12741 (to C.H.) and CA26423 (to B.J.O.) from the National Cancer Institute, HHS, the Eye Research Foundation of Missouri, Inc., and the Kansas Agricultural Experiment Station
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Abbreviations
- Py3T3:
-
polyoma-virus-transformed 3T3 mouse fibroblasts
- TMV:
-
tobacco mosaic virus
- KA31:
-
murine-sarcoma-virus-transformed Balb/3T3 mouse fibroblasts
- BD cellulose:
-
benzoylated DEAE cellulose
- pm7G:
-
N-7-methylguanosine 5′-phosphate
- pUm:
-
2′-O-methyluridine 5′-phosphate (and other nucleoside 5′-phosphates are indicated similarly as pN)
- s2mcm5U:
-
2-thio-5-methylcarboxymethyluridine
- t6A:
-
N-[9-(ß-D-ribofuranosylpurin-6-yl)carbamoyl] threonine
- ms2t6A:
-
the 2-methylthio derivative of t6A
- A260 unit:
-
the amount of tRNA in 1 ml aqueous solution that gives an absorbance of 1 for a 1-cm light path and is about 40 µg tRNA when measured in water
- SDS:
-
sodium dodecylsulfate
- PEI cellulose:
-
polyethyleneimine-im-pregnated cellulose
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Hedgcoth, C. et al. (1983). Perturbation of the Mitochondrial Lysine tRNA Population by Virus-Induced Transformation or Stress of Mammalian Cells: Functional Properties and Nucleotide Sequence of a Mitochondrially Associated Lysine tRNA. In: Nass, G. (eds) Modified Nucleosides and Cancer. Recent Results in Cancer Research/Fortschritte der Krebsforschung/Progrès dans les recherches sur Ie cancer, vol 84. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-81947-6_12
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