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Why Is Tumor tRNA Hypomodified with Respect to Q Nucleoside?

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Modified Nucleosides and Cancer

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Abstract

Nucleoside Q is found exclusively in the first position of the anticodon in tRNATyr, tRNAHis, tRNAAsn, and tRNAAsp (Kasai et al. 1975b, 1976). Its function is unknown, and long-term Q-deficient, germ-free mice appear normal (Reyniers et al. 1981). However, queuirie metabolism is altered in neoplasia. Many tumors exhibit Q-deficient tRNA (Katze 1975; Okada et al. 1978; Roe et al. 1979) and queuine addition to tumor-bearing mice can reverse tumor tRNA Q deficiency (Katze and Beck 1980; Nishimura et al., this volume pp. 401–412) and may inhibit tumor growth (Katze and Beck 1980). It is unlikely that Q-deficient tRNA is necessary for the neoplastic state since the careful study of Marini and Mushinski (1979; Mushinski and Marini, this volume pp. 121–132) shows widely varying, repeatable isoaccepting patterns (largely due to differences in Q content) for the Q family of tRNAs from several Balb/c mouse plasmacytomas with similar growth rates and size at harvest. These large differences among such a similar group of tumors suggest that the alteration in Q metabolism is secondary to the neoplastic process.

This work was supported by grants from the National Institutes of Health, CA-23099 and CA-21765 (W.T.B.), CA-20919 (J.R.K.), GM-29812 (J.A.M.); and ALSAC (W.T.B.). We thank P. Moore for her excellent assistance

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Abbreviations

Q or nucleoside Q or queuosine:

7-(4,5-cis-dihydroxy-l-cyclopenten-3-ylaminomethyl)-7-deaza-guanosine

Q base or queuine:

the aglycone of queuosine

[Q+]tRNA:

tRNA containing Q in the first position of the anticodon

[Q-]tRNA:

the unmodified or precursor form of [Q+]tRNA containing guanosine instead of Q in the first position of the anticodon

rQD3 :

the reduced and deuterated derivative of queuine (see text)

rQT3 :

the reduced and tritiated derivative of queuine (see text)

8azaG:

8-azaguanine

FBS:

fetal bovine serum

[Q-]FBS:

FBS treated with charcoal plus dextran to remove queuine (Katze, 1978b)

HPLC:

high-performance liquid chromatography

HEPES:

N-2-hydroxyethylpiperazine-N′-2ethane sulfonic acid

Tricine:

N-Tris(hydroxymethyl)methyl glycine

A260 unit:

the amount of material giving an absorbance of 1.0 at 260 nm when dissolved in 1 ml of water and measured in a cell of a 1-cm light path. In this study, Q is used generically to designate Q, and Q* denotes queuosine containing galactose or mannose bound to carbon 4 of the cyclopentenediol substituent.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, University of Tennessee Center for the Health Sciences, Memphis, TN, USA

    J. R. Katze

  2. Division of Biochemical and Clinical Pharmacology, St. Jude Children’s Research Hospital, USA

    W. T. Beck

  3. Department of Pharmacology, University of Tennessee Center for the Health Sciences, Memphis, TN, USA

    W. T. Beck

  4. Departments of Medicinal Chemistry and Biochemistry, University of Utah, Salt Lake City, UT, USA

    C. S. Cheng & J. A. McCloskey

Authors
  1. J. R. Katze
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  2. W. T. Beck
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  3. C. S. Cheng
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  4. J. A. McCloskey
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Editor information

Editors and Affiliations

  1. Zentrallaboratorium für Mutagenitätsprüfung, Deutschen Forschungsgemeinschaft, Breisacher Strasse 33, D-7800, Freiburg, Germany

    Gisela Nass

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© 1983 Springer-Verlag Berlin • Heidelberg

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Katze, J.R., Beck, W.T., Cheng, C.S., McCloskey, J.A. (1983). Why Is Tumor tRNA Hypomodified with Respect to Q Nucleoside?. In: Nass, G. (eds) Modified Nucleosides and Cancer. Recent Results in Cancer Research/Fortschritte der Krebsforschung/Progrès dans les recherches sur Ie cancer, vol 84. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-81947-6_10

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  • DOI: https://doi.org/10.1007/978-3-642-81947-6_10

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