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Nonfrozen Storage of Plant Cell Cultures and Its Effect on Metabolites

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Book cover Medicinal and Aromatic Plants I

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 4))

Abstract

Cultured plant cells are widely used as experimental tools in the fields of plant physiology, biochemistry, pathology, genetics, horticulture, and agriculture (Bajaj 1986a). Callus tissues or cell suspensions can be maintained by serial subculture. However, genetic changes of stock cultures are generally inevitable during sub-culturing (D’Amato 1977). Various methods of reducing growth rates have been applied for the purpose of minimal-growth storage of cultured cells to diminish deterioration of valuable culture strains, e.g., mineral oil overlay (Caplin 1959), low pressure and low oxygen (Bridgen and Staby 1981), sucrose-free medium (Jones 1974), dehydration (Nitzsche 1978, 1980), the addition of abscisic acid (ABA) or mannitol to the medium (Henshaw et al. 1978) and low temperature storage (Bajaj 1986b).

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Authors and Affiliations

  1. Niigata College of Pharmacy, 5-13-2 Kamishinei-cho, Niigata, 950-21, Japan

    N. Hiraoka

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  1. N. Hiraoka
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Editors and Affiliations

  1. A-137, New Friends Colony, New Delhi, 110065, India

    Y. P. S. Bajaj

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© 1988 Springer-Verlag Berlin Heidelberg

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Hiraoka, N. (1988). Nonfrozen Storage of Plant Cell Cultures and Its Effect on Metabolites. In: Bajaj, Y.P.S. (eds) Medicinal and Aromatic Plants I. Biotechnology in Agriculture and Forestry, vol 4. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73026-9_7

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  • DOI: https://doi.org/10.1007/978-3-642-73026-9_7

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-73028-3

  • Online ISBN: 978-3-642-73026-9

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