Zusammenfassung
Prinzipiell neue Verfahren zur Isolierung, quantitativen Bestimmung und Identifizierung von Indolderivaten sind im Berichtsjahr nicht vorgelegt worden. Dennoch muß auf einige wesentliche Verbesserungen vornehmlich dünnschichtchromatographischer Methoden hingewiesen werden. So erlaubt ein neu entwickeltes zweidimensionales Laufmittelsystem (HAWORTH u. WALMSLEY) die eindeutige Trennung von 32 Tryptophanabkömmlingen auf Kieselgelschichten. Die gute Elektronen-Donatoreigenschaft des Indolrings in “charge-transfer-Komplexen” eignet sich zur Komplexbildung mit organischen Elektronenacceptoren unmittelbar auf DC-Platten. Die dabei auftretenden, spezifisch gefärbten Komplexe können im Bereich von 0,5 – 10 µg durch Reflektionsmessung direkt bestimmt oder zur massenspektrometrischen Analyse isoliert werden (HUTZINGER et al.). Fluorescensspektroskopische Verfahren erlauben eine qualitative Identifizierung DC-getrennter Indolderivate sogar bis herab zu 0,03 – 0,05 µg (MACNEILL et al.). Spezifische Verschiebungen, die im UV-Spektrum bei 3-substituierten Indolderivaten in konz. Mineralsäuren auftreten, werden von MOLLAN et al. (1) zur Identifizierung und Messung nativer Indole herangezogen.
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Schraudolf, H. (1973). Wachstum. In: Ellenberg, H., Esser, K., Merxmüller, H., Schnepf, E., Ziegler, H. (eds) Fortschritte der Botanik. Fortschritte der Botanik, vol 35. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-65736-8_11
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