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Release of Monoamines in the Central Nervous System

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New Aspects of Storage and Release Mechanisms of Catecholamines

Part of the book series: Bayer Symposium ((BAYER-SYMP,volume 2))

Abstract

During the last few years, changes in turnover of monoamines in the central nervous system (CNS) have been observed in various pharmacological or physiological situations. These turnover estimations, obtained with the help of amine synthesis inhibitors or by labelling the central aminergic neurons, allowed the detection of large modifications in the rate of global utilization of monoamines. But, changes in turnover do not provide direct and precise information on the rapid fluctuations of quantities of amines released from terminals in central synapses, and thus available at receptor sites Limitations in the sensitivity of the spectrofluorimetric techniques used to estimate monoamines and practical difficulties to collect amines released from nerve terminals in deep structures of the CNS explain the few attempts which have been made to directly evaluate the release of monoamines (Vogt, 1969). Nevertheless, we have tried during the last 2 years in in vivo or in vitro studies, to demonstrate the release of radioactive Catecholaminess (CA) or serotonin (5-HT) synthesized from their original precursors: 3H tyrosine and 3H tryptophane respectively. Precursors of high specific activity were used in order to detect very small amounts of 3H amines released (≠ 5 pg). Besides its high sensitivity, this experimental approach offers three main advantages:

  1. 1.

    The 3H amines released spontaneously or after activation of aminergic neurons are liberated, as the respective endogenous amines, specifically from Catecholamines or 5-HT containing neurons.

  2. 2.

    The relationships between release and biosynthesis processes occurring during or after the neurons activation can be studied dynamically.

  3. 3.

    Finally, the involvement of the different intraneuronal storage forms of monoamines in the processes of spontaneous or evoked release of transmitters from nerve terminals can be examined and evaluated.

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Authors and Affiliations

  1. Laboratoire de Neurophysiologie Generale Unite de Neuropharmacologie Biochimique, College de France, 11 Place Marcelin-Berthelot, F-75, Paris V, France

    Dr. J. Glowinski

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  1. Dr. J. Glowinski
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Editors and Affiliations

  1. Klinikum Essen, Ruhruniversität Bochum, 4300, Essen-Holsterhausen, Deutschland

    Hans-Joachim Schümann (Direktor des Pharmakologischen Institutes) (Direktor des Pharmakologischen Institutes)

  2. Farbenfabriken Bayer AG., 5600, Wuppertal-Elberfeld, Deutschland

    Günter Kroneberg (Vorstand des Institutes für Pharmakologie) (Vorstand des Institutes für Pharmakologie)

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© 1970 Springer-Verlag

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Glowinski, J. (1970). Release of Monoamines in the Central Nervous System. In: Schümann, HJ., Kroneberg, G. (eds) New Aspects of Storage and Release Mechanisms of Catecholamines. Bayer Symposium, vol 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-49747-6_21

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  • DOI: https://doi.org/10.1007/978-3-642-49747-6_21

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-49465-9

  • Online ISBN: 978-3-642-49747-6

  • eBook Packages: Springer Book Archive

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